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      Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae) 1


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          Premise of the study:

          Microsatellite loci were isolated in Hypolepis punctata (Dennstaedtiaceae) to further study the reproductive ecology of this species.

          Methods and Results:

          We developed 16 microsatellite loci from one sample of H. punctata using an enriched genomic library. These loci were characterized in 28 individuals. The number of alleles per locus ranged from two to 10, and the expected heterozygosity ranged from 0.036 to 0.845.


          The results indicate that the microsatellite markers can facilitate further studies on inferring the phylogeography and population genetics of H. punctata and related species.

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          Most cited references2

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          Isolating microsatellite DNA loci.

          A series of techniques are presented to construct genomic DNA libraries highly enriched for microsatellite DNA loci. The individual techniques used here derive from several published protocols but have been optimized and tested in our research laboratories as well as in classroom settings at the University of South Carolina and University of Georgia, with students achieving nearly 100% success. Reducing the number of manipulations involved has been a key to success, decreasing both the failure rate and the time necessary to isolate loci of interest. In our lab during the past 3 years alone, these protocols have been successfully used to isolate microsatellite DNA loci from at least 55 species representing three kingdoms. These protocols have made it possible to reduce the time to identify candidate loci for primer development from most eukaryotic species to as little as 1 week.
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            Reproductive and competitive interactions among gametophytes of the allotetraploid fern Dryopteris corleyi and its two diploid parents.

            Several models predict that the establishment of polyploids within diploid populations is enhanced by non-random mating (i.e. selfing and assortative mating) of cytotypes and by a higher relative fitness of polyploids. This report assesses the role that antheridiogens (i.e. maleness-inducing pheromones) and intercytotype differences in growth rate have on polyploid performance.

              Author and article information

              Appl Plant Sci
              Appl Plant Sci
              Applications in Plant Sciences
              Botanical Society of America
              September 2015
              4 September 2015
              : 3
              : 9
              : apps.1500047
              [2 ]Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, People’s Republic of China
              [3 ]College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, People’s Republic of China
              Author notes

              The authors thank C.-X. Wang, H.-J. Wei, Y.-F. Gu, and X.-L. Zhou for their assistance in field surveys and sample collection. This study was supported by the Shanghai Municipal Administration of Forestation and City Appearances (grant no. G152419 and F132421).

              [4 ]Author for correspondence: yhyan@ 123456sibs.ac.cn
              © 2015 Shang et al. Published by the Botanical Society of America

              This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).

              : 27 April 2015
              : 4 June 2015
              Primer Note

              dennstaedtiaceae,hypolepis punctata,microsatellite,phylogeography,population genetics


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