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      Two Rab GTPases play different roles in conidiation, trap formation, stress resistance, and virulence in the nematode-trapping fungus Arthrobotrys oligospora

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          Protein Identification and Analysis Tools on the ExPASy Server

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            Multifunctional yeast high-copy-number shuttle vectors.

            A set of four yeast shuttle vectors that incorporate sequences from the Saccharomyces cerevisiae 2 mu endogenous plasmid has been constructed. These yeast episomal plasmid (YEp)-type vectors (pRS420 series) differ only in their yeast selectable markers, HIS3, TRP1, LEU2 or URA3. The pRS420 plasmids are based on the backbone of a multifunctional phagemid, pBluescript II SK+, and share its useful properties for growth in Escherichia coli and manipulation in vitro. The pRS420 plasmids have a copy number of about 20 per cell, equivalent to that of YEp24. During non-selective yeast growth, pRS420 plasmids are lost through mitotic segregation at rates similar to other YEp vectors and yeast centromeric plasmid (YCp) vectors, in the range of 1.5-5% of progeny per doubling. The pRS420 series provides high-copy-number counterparts to the current pRS vectors [Sikorski and Hieter, Genetics 122 (1989) 19-27].
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              Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C.

              A set of GAL2+ yeast strains that are isogenic to strain S288C have been constructed. They contain non-reverting mutations in genes commonly used for selection for recombinant plasmids. Strains from this collection are being used for the European Union Yeast Genome Sequencing Programme. Representative strains from this collection have been deposited with the ATCC.
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                Author and article information

                Journal
                Applied Microbiology and Biotechnology
                Appl Microbiol Biotechnol
                Springer Nature
                0175-7598
                1432-0614
                May 2018
                April 3 2018
                May 2018
                : 102
                : 10
                : 4601-4613
                Article
                10.1007/s00253-018-8929-1
                29616315
                2baf22fb-5945-4c5a-ad20-b6b80b789c90
                © 2018

                http://www.springer.com/tdm

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