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      Effect of small interfering RNA targeting wild-type FLT3 in acute myeloid leukaemia cells in vitro and in vivo.

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          Abstract

          This study investigated the effect of using small interfering RNA (siRNA) to silence the wild-type FMS-like tyrosine kinase 3 (FLT3) gene in acute myeloid leukaemia (AML) cells, in vitro and in vivo. FLT3 siRNA was introduced into the human AML cell line, THP1, and into a THP1 xenograft tumour model in BALB/c nude mice. FLT3 siRNA effectively reduced both the mRNA and the protein levels of FLT3, arrested cells in G(0)/G(1) phase, inhibited THP1 cell proliferation and increased apoptosis. Intraperitoneal injection of FLT3 siRNA suppressed tumour growth in BALB/c nude mice. FLT3 siRNA treatment also reduced cyclin D1 and Bcl-2 protein levels, and increased the nuclear level of silencing mediator for retinoic acid and thyroid hormone receptors protein both in vitro and in vivo. These data suggest that FLT3 siRNA is a strong inhibitor of FLT3 expression in vitro and in vivo, and may provide a new therapeutic target for AML.

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          Author and article information

          Journal
          J. Int. Med. Res.
          The Journal of international medical research
          SAGE Publications
          1473-2300
          0300-0605
          2011
          : 39
          : 5
          Affiliations
          [1 ] Department of Paediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
          Article
          10.1177/147323001103900508
          22117966
          2bce34ce-1a64-4431-bce3-2f3d23acb072
          History

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