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      Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell.

      Nature medicine

      Adult Stem Cells, metabolism, Animals, Body Weight, physiology, Cells, Cultured, Chromogranin A, Colon, cytology, transplantation, Cyclooxygenase 1, DNA-Binding Proteins, deficiency, Epithelium, Green Fluorescent Proteins, genetics, In Vitro Techniques, Ki-67 Antigen, Membrane Proteins, Mice, Mice, Transgenic, Organoids, Receptors, G-Protein-Coupled, Rhodamines, Time Factors, Transplants

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          Adult stem-cell therapy holds promise for the treatment of gastrointestinal diseases. Here we describe methods for long-term expansion of colonic stem cells positive for leucine-rich repeat containing G protein-coupled receptor 5 (Lgr5(+) cells) in culture. To test the transplantability of these cells, we reintroduced cultured GFP(+) colon organoids into superficially damaged mouse colon. The transplanted donor cells readily integrated into the mouse colon, covering the area that lacked epithelium as a result of the introduced damage in recipient mice. At 4 weeks after transplantation, the donor-derived cells constituted a single-layered epithelium, which formed self-renewing crypts that were functionally and histologically normal. Moreover, we observed long-term (>6 months) engraftment with transplantation of organoids derived from a single Lgr5(+) colon stem cell after extensive in vitro expansion. These data show the feasibility of colon stem-cell therapy based on the in vitro expansion of a single adult colonic stem cell.

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