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      Molecular interaction studies using microscale thermophoresis.

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          Abstract

          Abstract The use of infrared laser sources for creation of localized temperature fields has opened new possibilities for basic research and drug discovery. A recently developed technology, Microscale Thermophoresis (MST), uses this temperature field to perform biomolecular interaction studies. Thermophoresis, the motion of molecules in temperature fields, is very sensitive to changes in size, charge, and solvation shell of a molecule and thus suited for bioanalytics. This review focuses on the theoretical background of MST and gives a detailed overview on various applications to demonstrate the broad applicability. Experiments range from the quantification of the affinity of low-molecular-weight binders using fluorescently labeled proteins, to interactions between macromolecules and multi-component complexes like receptor containing liposomes. Information regarding experiment and experimental setup is based on the Monolith NT.115 instrument (NanoTemper Technologies GmbH).

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          Author and article information

          Journal
          Assay Drug Dev Technol
          Assay and drug development technologies
          Mary Ann Liebert Inc
          1557-8127
          1540-658X
          Aug 2011
          : 9
          : 4
          Affiliations
          [1 ] Systems Biophysics, Center for Nanoscience, Munich, Germany.
          Article
          10.1089/adt.2011.0380
          3148787
          21812660
          2bd736df-fd55-4e43-ad8e-6c3559902a03
          History

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