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      Identification of scFv antibody fragments that specifically recognise the heroin metabolite 6-monoacetylmorphine but not morphine

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          Abstract

          Use of phage display of recombinant antibodies and large repertoire naïve antibody libraries for identifying antibodies of high specificity has been extensively reported. Nevertheless, there have been few reported antibodies to haptens that have originated from naïve antibody libraries with potential use in diagnostics. We have used chain shuffling of lead single-chain fragment variable (scFv) antibodies, isolated from a naïve antibody library, to screen for antibodies that specifically recognise the major metabolite of heroin, 6-monoacetylmorphine (6MAM). The antibodies were identified by screening high-density colonies of Escherichia coli expressing soluble scFv antibody fragments without prior expression on bacteriophage (phage display). The antibodies recognise 6MAM with affinities of 1-3x10(-7) M with no crossreactivity to morphine. These antibodies can potentially be used for developing a rapid immunoassay in drug-testing programs. To our knowledge, this is the first report of an antibody that distinguishes 6MAM from its de-acetylated form, morphine.

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          Author and article information

          Journal
          Journal of Immunological Methods
          Journal of Immunological Methods
          Elsevier BV
          00221759
          September 2003
          September 2003
          : 280
          : 1-2
          : 139-155
          Article
          10.1016/S0022-1759(03)00109-1
          12972195
          2bf964ac-f6f6-4974-b517-13fef1e1e540
          © 2003

          https://www.elsevier.com/tdm/userlicense/1.0/

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