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      Draft Genome Sequence of Escherichia coli DSM 12242, a Highly Efficient Host Strain for the Isolation of Somatic Coliphages

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          Abstract

          Here, we report the draft genome sequence and characterization of the commercial strain Escherichia coli DSM 12242 (=ATCC 13706/60 =NZRM 3262) derived from strain C (ATCC 13706), which is suitable for the isolation of coliphages from environmental and clinical samples.

          ABSTRACT

          Here, we report the draft genome sequence and characterization of the commercial strain Escherichia coli DSM 12242 (=ATCC 13706/60 =NZRM 3262) derived from strain C (ATCC 13706), which is suitable for the isolation of coliphages from environmental and clinical samples.

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          Bacteriophages Isolated from Chicken Meat and the Horizontal Transfer of Antimicrobial Resistance Genes.

          Amira Shousha, Nattakarn Awaiwanont, Dmitrij Sofka … (2015)
          Antimicrobial resistance in microbes poses a global and increasing threat to public health. The horizontal transfer of antimicrobial resistance genes was thought to be due largely to conjugative plasmids or transposons, with only a minor part being played by transduction through bacteriophages. However, whole-genome sequencing has recently shown that the latter mechanism could be highly important in the exchange of antimicrobial resistance genes between microorganisms and environments. The transfer of antimicrobial resistance genes by phages could underlie the origin of resistant bacteria found in food. We show that chicken meat carries a number of phages capable of transferring antimicrobial resistance. Of 243 phages randomly isolated from chicken meat, about a quarter (24.7%) were able to transduce resistance to one or more of the five antimicrobials tested into Escherichia coli ATCC 13706 (DSM 12242). Resistance to kanamycin was transduced the most often, followed by that to chloramphenicol, with four phages transducing tetracycline resistance and three transducing ampicillin resistance. Phages able to transduce antimicrobial resistance were isolated from 44% of the samples of chicken meat that we tested. The statistically significant (P = 0.01) relationship between the presence of phages transducing kanamycin resistance and E. coli isolates resistant to this antibiotic suggests that transduction may be an important mechanism for transferring kanamycin resistance to E. coli. It appears that the transduction of resistance to certain antimicrobials, e.g., kanamycin, not only is widely distributed in E. coli isolates found on meat but also could represent a major mechanism for resistance transfer. The result is of high importance for animal and human health.
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            A simple membrane filtration-elution method for the enumeration of F-RNA, F-DNA and somatic coliphages in 100-ml water samples

            A.J Reid, L.W. Sinton, R.K. Finlay (1996)
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              Antimicrobial Resistance-Transducing Bacteriophages Isolated from Surfaces of Equine Surgery Clinics – A Pilot Study

              Miriam Hilbert, Isabella Csadek, Ulrike Auer … (2017)
              In the past, the horizontal transfer of antimicrobial resistance genes was mainly associated with conjugative plasmids or transposons, whereas transduction by bacteriophages was thought to be a rare event. In order to analyze the likelihood of transduction of antimicrobial resistance in the field of clinical veterinary medicine, we isolated phages from Escherichia coli from a surgery suite of an equine clinic. In a pilot study, the surgery suite of a horse clinic was sampled directly after surgery and subsequently sampled after cleaning and disinfection following a sampling plan based on hygiene, surgery, and anesthesia. In total, 31 surface sampling sites were defined and sampled. At 24 of these 31 surface sampling sites, coliphages were isolated. At 12 sites, coliphages were found after cleaning and disinfection. Randomly selected phages were tested for their ability of antimicrobial resistance transduction. Ten of 31 phages were detected to transfer antimicrobial resistance. These phages most often transduced resistance to streptomycin, encoded by the addA1 gene (n = 9), followed by resistance to chloramphenicol by cmlA (n = 3) and ampicillin (n = 1). This is, to the best of our knowledge, the first report on antimicrobial resistance-transferring bacteriophages that have been isolated at equine veterinary clinics.
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                Author and article information

                Contributors
                Catherine Putonti: Role: Editor
                Journal
                Journal ID (nlm-ta): Microbiol Resour Announc
                Journal ID (iso-abbrev): Microbiol Resour Announc
                Journal ID (hwp): ga
                Journal ID (pmc): mra
                Journal ID (publisher-id): MRA
                Title: Microbiology Resource Announcements
                Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
                ISSN (Electronic): 2576-098X
                Publication date (Electronic): 19 September 2019
                Publication date Collection: September 2019
                Volume: 8
                Issue: 38
                Electronic Location Identifier: e00953-19
                Affiliations
                [a ]Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria
                [b ]Karl Landsteiner University of Health Sciences, Krems an der Donau, Austria
                [c ]Instituto de Medicina Molecular, Faculty of Medicine, University of Lisbon, Lisbon, Portugal
                Loyola University Chicago
                Author notes
                Address correspondence to Cátia Pacífico, 1545365@ 123456students.vetmeduni.ac.at .

                All authors contributed equally to this work.

                Citation Pacífico C, Sofka D, Carriço JA, Hilbert F. 2019. Draft genome sequence of Escherichia coli DSM 12242, a highly efficient host strain for the isolation of somatic coliphages. Microbiol Resour Announc 8:e00953-19. https://doi.org/10.1128/MRA.00953-19.

                Author information
                https://orcid.org/0000-0002-6278-5777
                http://orcid.org/0000-0002-5274-2722
                Article
                Publisher ID: MRA00953-19
                DOI: 10.1128/MRA.00953-19
                PMC ID: 6753275
                PubMed ID: 31537671
                SO-VID: 2c5378e6-0bb2-4811-9902-c20ca9334d98
                Copyright © Copyright © 2019 Pacífico et al.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

                History
                Date received : 6 August 2019
                Date accepted : 27 August 2019
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 20, Pages: 2, Words: 1514
                Funding
                Funded by: NFB;
                Award ID: LS14-006
                Award Recipient :
                Categories
                Subject: Genome Sequences
                Custom metadata
                cover-date September 2019

                Data availability:

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