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      Cloning and characterization of cDNA encoding a precursor for human adrenomedullin.

      Biochemical and Biophysical Research Communications

      Transcription, Genetic, Adrenal Gland Neoplasms, Restriction Mapping, isolation & purification, genetics, biosynthesis, RNA, Neoplasm, RNA, Messenger, RNA, Protein Precursors, Poly A, metabolism, Pheochromocytoma, Peptides, Peptide Biosynthesis, Organ Specificity, Molecular Sequence Data, Humans, DNA, Neoplasm, Cloning, Molecular, Base Sequence, Antihypertensive Agents, Amino Acid Sequence, Adrenomedullin, Adrenal Medulla

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          Abstract

          Adrenomedullin is a novel hypotensive peptide recently isolated from human pheochromocytoma. Since a high concentration of immunoreactive adrenomedullin was found in pheochromocytoma tissue, the cDNA library of pheochromocytoma was constructed, and the cDNA clone encoding an adrenomedullin precursor was isolated and sequenced. The precursor for human adrenomedullin (human preproadrenomedullin) is 185 amino acids in length, including an adrenomedullin sequence. Proadrenomedullin (proAM) contains a unique twenty amino acid sequence followed by Gly-Lys-Arg in the N-terminal region. It is possible that a novel 20 residues peptide, termed "proadrenomedullin N-terminal 20 peptide" (proAM-N20) whose carboxy terminus may be Arg-NH2, is processed from proadrenomedullin. By RNA blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues including adrenal medulla, ventricle, lung and kidney as well as pheochromocytoma.

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          Journal
          10.1006/bbrc.1993.1881
          7688224

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