The RNA helicase Rho triggers 20–30% of transcription termination events in bacteria. While Rho is associated with most transcription elongation complexes, it only promotes termination of a subset. Recent studies of individual Rho-dependent terminators located within the 5′ leader regions of bacterial mRNAs have identified novel mechanisms that govern Rho target specificity and revealed unanticipated physiological functions for Rho. In particular, the multistep nature of Rho-dependent termination enables regulatory input from determinants beyond the sequence of the Rho loading site and allows a given Rho-dependent terminator to respond to multiple signals. Further, the unique position of Rho as a sensor of cellular translation has been exploited to regulate transcription of genes required for protein synthesis, including those specifying Mg 2+ transporters.