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      Inflammatory mediator-induced modulation of GABAA currents in human sensory neurons

      , , , ,

      Neuroscience

      Elsevier BV

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          Abstract

          <p class="first" id="P3">The purpose of the present study was to characterize the properties of GABA <sub>A</sub> receptor currents in human sensory neurons. Neurons were obtained from adult organ donors. GABA <sub>A</sub> currents were recorded in isolated neurons. Both large inactivating low affinity currents and smaller persistent high affinity currents were present in all of the 129 neurons studied from 15 donors. The kinetics of human GABA <sub>A</sub> currents were slower than those in rat sensory neurons. GABA currents were completely blocked by bicuculline (10 µM), and persistent currents were activated by the δ-subunit preferring agonist, THIP. The GABA current equilibrium potential was ~20 mV more hyperpolarized than in rat neurons. Both low and high affinity currents were increased by inflammatory mediators but via different second messenger pathways. These results highlight potentially important species differences in the properties of ion channels present in their native environment and suggest the use of human sensory neurons may be a valuable tool to test compounds prior to use in humans. </p>

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          Author and article information

          Journal
          Neuroscience
          Neuroscience
          Elsevier BV
          03064522
          December 2015
          December 2015
          : 310
          : 401-409
          Article
          10.1016/j.neuroscience.2015.09.048
          4633346
          26415765
          © 2015

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