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      Two new combinations in Oreocharis (Gesneriaceae) based on morphological, molecular and cytological evidence

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          Abstract

          The newly-circumscribed genus Oreocharis is recently enlarged by incorporating ten other genera with high floral diversity. In this study, our morphological, molecular and cytological evidence supports our adding two species from other two different genera ( Boeica and Beccarinda ) to Oreocharis . The special corolla shape (campanulate or flat-faced) and related short filament of these two new combinations, Oreocharis guileana and O. baolianis , further enrich the diversity of floral characters of the enlarged Oreocharis . Meanwhile, some supplementary and amended descriptions of these two species are made here. Our morphological, molecular and geographical data indicate that O. guileana is related to O. pilosopetiolata to a certain extent. For O. baolianis , however, our current dataset does not allow conclusions on the species relationship within Oreocharis .

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          Most cited references28

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          SequenceMatrix: concatenation software for the fast assembly of multi-gene datasets with character set and codon information

          We present SequenceMatrix, software that is designed to facilitate the assembly and analysis of multi-gene datasets. Genes are concatenated by dragging and dropping FASTA, NEXUS, or TNT files with aligned sequences into the program window. A multi-gene dataset is concatenated and displayed in a spreadsheet; each sequence is represented by a cell that provides information on sequence length, number of indels, the number of ambiguous bases ("Ns"), and the availability of codon information. Alternatively, GenBank numbers for the sequences can be displayed and exported. Matrices with hundreds of genes and taxa can be concatenated within minutes and exported in TNT, NEXUS, or PHYLIP formats, preserving both character set and codon information for TNT and NEXUS files. SequenceMatrix also creates taxon sets listing taxa with a minimum number of characters or gene fragments, which helps assess preliminary datasets. Entire taxa, whole gene fragments, or individual sequences for a particular gene and species can be excluded from export. Data matrices can be re-split into their component genes and the gene fragments can be exported as individual gene files. SequenceMatrix also includes two tools that help to identify sequences that may have been compromised through laboratory contamination or data management error. One tool lists identical or near-identical sequences within genes, while the other compares the pairwise distance pattern of one gene against the pattern for all remaining genes combined. SequenceMatrix is Java-based and compatible with the Microsoft Windows, Apple MacOS X and Linux operating systems. The software is freely available from http://code.google.com/p/sequencematrix/. © The Willi Hennig Society 2010.
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            TESTING SIGNIFICANCE OF INCONGRUENCE

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              A rapid DNA isolation procedure for small quantities of fresh leaf tissues

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                Author and article information

                Contributors
                Journal
                PhytoKeys
                PhytoKeys
                3
                urn:lsid:arphahub.com:pub:F7FCE910-8E78-573F-9C77-7788555F8AAD
                PhytoKeys
                Pensoft Publishers
                1314-2011
                1314-2003
                2020
                26 August 2020
                : 157
                : 43-58
                Affiliations
                [1 ] Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, CN-510650, China Chinese Academy of Sciences Guangzhou China
                [2 ] Guangxi Key Laboratory of Plant Conservation and Restoration Ecology in Karst Terrain, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, CN-541006, China Guangxi Institute of Botany Guilin China
                [3 ] University of Chinese Academy of Sciences, Yuquan Road, Shijingshan District, Beijing, CN-100049, China University of Chinese Academy of Sciences Beijing China
                [4 ] College of Life Sciences, Guangxi Normal University, Guilin, CN-541004, China Guangxi Normal University Guilin China
                Author notes
                Corresponding author: Ming Kang ( mingkang@ 123456scbg.ac.cn )

                Academic editor: Y.-M. Shui

                Author information
                https://orcid.org/0000-0002-3889-8835
                https://orcid.org/0000-0003-2721-0449
                Article
                32609
                10.3897/phytokeys.157.32609
                7467946
                32934447
                2d3f3b33-360e-4e06-8de1-83418b9508ef
                Li-Hua Yang, Fang Wen, Hang-Hui Kong, Zhi-Xia Sun, Lan-Ying Su, Ming Kang

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 21 December 2018
                : 13 May 2020
                Categories
                Research Article
                Gesneriaceae

                Plant science & Botany
                beccarinda baolianis,boeica guileana,its, oreocharis ,taxonomy,trnl-f,plantae,passeriformes,paramythiidae

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