29
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Bmp7 Regulates the Survival, Proliferation, and Neurogenic Properties of Neural Progenitor Cells during Corticogenesis in the Mouse

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Bone morphogenetic proteins (BMPs) are considered important regulators of neural development. However, results mainly from a wide set of in vitro gain-of-function experiments are conflicting since these show that BMPs can act either as inhibitors or promoters of neurogenesis. Here, we report a specific and non-redundant role for BMP7 in cortical neurogenesis in vivo using knockout mice. Bmp7 is produced in regions adjacent to the developing cortex; the hem, meninges, and choroid plexus, and can be detected in the cerebrospinal fluid. Bmp7 deletion results in reduced cortical thickening, impaired neurogenesis, and loss of radial glia attachment to the meninges. Subsequent in vitro analyses of E14.5 cortical cells revealed that lack of Bmp7 affects neural progenitor cells, evidenced by their reduced proliferation, survival and self-renewal capacity. Addition of BMP7 was able to rescue these proliferation and survival defects. In addition, at the developmental stage E14.5 Bmp7 was also required to maintain Ngn2 expression in the subventricular zone. These data demonstrate a novel role for Bmp7 in the embryonic mouse cortex: Bmp7 nurtures radial glia cells and regulates fundamental properties of neural progenitor cells that subsequently affect Ngn2-dependent neurogenesis.

          Related collections

          Most cited references48

          • Record: found
          • Abstract: not found
          • Article: not found

          The cell biology of neurogenesis.

          During the development of the mammalian central nervous system, neural stem cells and their derivative progenitor cells generate neurons by asymmetric and symmetric divisions. The proliferation versus differentiation of these cells and the type of division are closely linked to their epithelial characteristics, notably, their apical-basal polarity and cell-cycle length. Here, we discuss how these features change during development from neuroepithelial to radial glial cells, and how this transition affects cell fate and neurogenesis.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Transcriptional control by the TGF-beta/Smad signaling system.

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              A protein related to extracellular matrix proteins deleted in the mouse mutant reeler.

              The autosomal recessive mouse mutation reeler leads to impaired motor coordination, tremors and ataxia. Neurons in affected mice fail to reach their correct locations in the developing brain, disrupting the organization of the cerebellar and cerebral cortices and other laminated regions. Here we use a previously characterized reeler allele (rl(tg)) to close a gene, reelin, deleted in two reeler alleles. Normal but not mutant mice express reelin in embryonic and postnatal neurons during periods of neuronal migration. The encoded protein resembles extracellular matrix proteins involved in cell adhesion. The reeler phenotype thus seems to reflect a failure of early events associated with brain lamination which are normally controlled by reelin.
                Bookmark

                Author and article information

                Contributors
                Role: Editor
                Journal
                PLoS One
                PLoS ONE
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, USA )
                1932-6203
                2012
                26 March 2012
                : 7
                : 3
                : e34088
                Affiliations
                [1 ]Institute of Immunology, Biomedical Sciences Research Center Alexander Fleming, Vari, Hellas-Greece
                [2 ]Laboratory of Molecular Biology (Celgen), Center for Human Genetics, K.U.Leuven, Leuven, Belgium
                [3 ]Department of Molecular and Developmental Genetics, VIB, K.U.Leuven, Leuven, Belgium
                [4 ]Institute of Molecular Biology and Genetics, Biomedical Sciences Research Center Alexander Fleming, Vari, Hellas-Greece
                [5 ]Faculty of Medicine, Institute of Oral Biology, University of Zurich, Zurich, Switzerland
                University of Nebraska Medical Center, United States of America
                Author notes

                Conceived and designed the experiments: DG ER E. Seuntjens. Performed the experiments: AS E. Seuntjens ME ST E. Stappers DG. Analyzed the data: AS E. Seuntjens ME ER DG. Contributed reagents/materials/analysis tools: DG ER DH. Wrote the paper: DG ER E. Seuntjens DH TAM.

                [¤]

                Current address: Department of Development and Regeneration, Faculty of Medicine, University of Leuven (K.U.Leuven), Leuven, Belgium

                Article
                PONE-D-11-18785
                10.1371/journal.pone.0034088
                3312908
                22461901
                2d6e60b6-05ce-4a9c-8db2-ea3a1f8705d5
                Segklia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
                History
                : 26 September 2011
                : 21 February 2012
                Page count
                Pages: 9
                Categories
                Research Article
                Biology
                Anatomy and Physiology
                Neurological System
                Developmental Biology
                Molecular Development
                Stem Cells
                Model Organisms
                Animal Models
                Molecular Cell Biology
                Neuroscience
                Developmental Neuroscience

                Uncategorized
                Uncategorized

                Comments

                Comment on this article