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      Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.

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      immunology, Animals, B-Lymphocytes, drug effects, enzymology, pathology, Chimera, Cytidine Deaminase, deficiency, genetics, metabolism, Cytokines, pharmacology, Enzyme Induction, Female, Flow Cytometry, Gene Deletion, Germ-Line Mutation, Germinal Center, Haptens, Heterozygote, Immunoglobulin A, Immunoglobulin Class Switching, Immunoglobulin M, Immunohistochemistry, Lipopolysaccharides, Lymphocyte Activation, Male, Mice, Mice, Knockout, Mutation, RNA Editing, RNA, Messenger, analysis, Recombination, Genetic, Spleen, Tumor Cells, Cultured, gamma-Globulins

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          Abstract

          Induced overexpression of AID in CH12F3-2 B lymphoma cells augmented class switching from IgM to IgA without cytokine stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. AID-/- spleen cells stimulated in vitro with LPS and cytokines failed to undergo class switch recombination although they expressed germline transcripts. Immunization of AID-/- chimera with 4-hydroxy-3-nitrophenylacetyl (NP) chicken gamma-globulin induced neither accumulation of mutations in the NP-specific variable region gene nor class switching. These results suggest that AID may be involved in regulation or catalysis of the DNA modification step of both class switching and somatic hypermutation.

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          11007474

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