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      Rapid colorimetric assay for cell growth and survival

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      Journal of Immunological Methods

      Elsevier BV

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          Most cited references 5

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          Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens.

           Ulf Landegren (1984)
          By using a chromogenic substrate for an ubiquitous lysosomal enzyme, hexosaminidase to estimate cell numbers, a sensitive and simple procedure has been developed in which microtiter reaction wells are directly scanned in a spectrophotometer. This method has been adapted to several cell biological assays. Quantitation of the biological activities of T cell growth factor and interferon can be performed on large numbers of samples. Adhesion of dispersed solid tissue cells to fibronectin coated substrates may be quantitated with little expenditure of reagents. By use of a panning procedure in microtiter plates a sensitive and very simple assay for the binding of monoclonal antibodies to cell surface antigens has been developed.
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            Studies on succinate-tetrazolium reductase systems

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              Monoclonal cytolytic T-cell lines

               KA Smith,  S Gillis,  PE Baker (1979)
              Monospecific cloned cytolytic T-lymphocyte lines have been created utilizing T-cell growth factor. The clones were found to retain their cytolytic specificity after prolonged culture and monospecific function was demonstrated by subcloning procedures. Thus, detailed studies of the phenotypic and functional characteristics of monospecific, homogeneous, cytolytic T lymphocytes will now be possible.
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                Author and article information

                Journal
                Journal of Immunological Methods
                Journal of Immunological Methods
                Elsevier BV
                00221759
                May 1986
                May 1986
                : 89
                : 2
                : 271-277
                Article
                10.1016/0022-1759(86)90368-6
                © 1986

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