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      Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes.

      Journal of Clinical Microbiology
      Animals, Base Sequence, DNA Primers, genetics, DNA, Protozoan, Genes, Protozoan, HSP70 Heat-Shock Proteins, Humans, Leishmania, classification, isolation & purification, Leishmaniasis, parasitology, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, methods, statistics & numerical data, Polymorphism, Restriction Fragment Length, Protozoan Proteins, Sensitivity and Specificity

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          Abstract

          PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

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