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      Effects of Temperature, Storage and Sodium Molybdate on the Analysis of Estrogen and Progesterone Receptors in Rabbit Uterine Tissue and Gynecologic Tumor

      Hormone Research in Paediatrics

      S. Karger AG

      Estrogen receptors, Progesterone receptors, Gynecologic tumors, Rabbit uterus, Molybdate

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          Abstract

          The cytoplasmic estrogen receptor (ER<sub>C</sub>) and progesterone receptor (PR<sub>C</sub>) in mammary tumors have been recognized as useful biochemical markers for predicting the objective response of patients with advanced breast cancers to endocrine therapy. These proteins are also useful in the prognosis of gynecologic carcinoma. This report presents data showing the effect of sodium molybdate in the stabilization of estrogen and progesterone receptors. In rabbit uterine tissue, molybdate (20 m M) increased the binding of progesterone and estrogen to the receptors in several ways: (a) the apparent loss of detectable receptors during lengthy sucrose gradient analysis and at elevated temperature (30°C) was reduced; (b) the instability of receptors due to storage at -70°C was lessened, and (c) the conversion of the 7 SPR<sub>C</sub> to the 3.5 S form was minimized. Similarly, molybdate caused a qualitative and/or a statistically significant quantitative difference in receptor values for some human gynecologic tumors presented herein; the molybdate-associated changes vary with tumor specimen. Of the 8 tumors for which receptor values in the presence of molybdate (M+) and its absence (M-) can be compared, detectable ER<sub>C</sub> of 6 and PR<sub>C</sub> of 7 tumors increased with molybdate, and ER<sub>C</sub> of 2 and PR<sub>C</sub> of 1 tumor showed no change. In addition to the increase in receptor values, a concomitant shift of the 3–4 S molecules to the 7–85 moieties was noted for some tumors (1 of 6 for ER<sub>C</sub> and 3 of 7 for PR<sub>C</sub>). In 2 receptor-poor tumor samples, ER<sub>C</sub> was only detected in M+ cytosols. These results show that molybdate is effective in reducing receptor degradation and stabilizes the 7–85’ molecules from converting to 4 S moieties. The addition of molybdate may be helpful for better quantitation of steroid receptors in clinical specimens.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1984
          1984
          26 November 2008
          : 19
          : 4
          : 243-252
          Affiliations
          Department of Obstetrics and Gynecology, University of Minnesota, Mayo Memorial Hospital, Minneapolis, Minn., USA
          Article
          179894 Horm Res 1984;19:243–252
          10.1159/000179894
          6745845
          © 1984 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 10
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