55
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      HOTAIR, a prognostic factor in esophageal squamous cell carcinoma, inhibits WIF‐1 expression and activates Wnt pathway

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Long non‐coding RNAs ( Lnc RNAs) have been recently found to be pervasively transcribed in the genome and critical regulators of the epigenome. HOTAIR, as a well‐known Lnc RNA, has been found to play important roles in several tumors. Herein, the clinical application value and biological functions of HOTAIR were focused and explored in esophageal squamous cell carcinoma ( ESCC). It was found that there was a great upregulation of HOTAIR in ESCC compared to their adjacent normal esophageal tissues. Meanwhile, patients with high HOTAIR expression have a significantly poorer prognosis than those with low expression. Moreover, HOTAIR was further validated to promote migration and invasion of ESCC cells in vitro. Then some specific molecules with great significance were investigated after HOTAIR overexpression using microarray and quantitative real time‐polymerase chain reaction ( qPCR). WIF‐1 playing an important role in Wnt/β‐catenin signaling pathway was selected and further tested by immunehistochemistry. Generally, inverse correlation between HOTAIR and WIF‐1 expression was demonstrated both in ESCC cells and tissues. Mechanistically, HOTAIR directly decreased WIF‐1 expression by promoting its histone H3 K27 methylation in the promoter region and then activated the Wnt/β‐catenin signaling pathway. This newly identified HOTAIR/ WIF‐1 axis clarified the molecular mechanism of ESCC cell metastasis and represented a novel therapeutic target in patients with ESCC.

          Abstract

          HOTAIR, a long non coding RNA, has been linked to the progression of several types of human cancer. In this study, we found that HOTAIR was not only significantly up‐regulated in tumor tissues, but also significantly associated with poor clinical outcome in patients with esophageal squamous cell carcinoma ( ESCC). ESCC cells with HOTAIR overexpression displayed aberrant activated WNT signaling pathway by inhibiting the expression of WNT‐inhibitor factor 1, thereby promote the migration and invasion of ESCC cells. On the contrary, silencing of HOTAIR in ESCC cells led to decreased migration and invasion ability. Together, these results suggest that HOTAIR overexpression may serve as a novel prognostic biomarker and potential therapeutic target for the treatment of human ESCC.

          Related collections

          Author and article information

          Journal
          Cancer Sci
          Cancer Sci
          10.1111/(ISSN)1349-7006
          CAS
          Cancer Science
          John Wiley and Sons Inc. (Hoboken )
          1347-9032
          1349-7006
          30 October 2013
          December 2013
          : 104
          : 12 ( doiID: 10.1111/cas.2013.104.issue-12 )
          : 1675-1682
          Affiliations
          [ 1 ] Department of Experimental Research State Key Laboratory of Oncology in South China Sun Yat‐Sen University Cancer Center Guangzhou China
          Author notes
          [*] [* ] To whom correspondence should be addressed.

          E‐mail: jiaweih@ 123456mail.sysu.edu.cn

          [†]

          These authors contributed equally to this work.

          Article
          PMC7653522 PMC7653522 7653522 CAS12296
          10.1111/cas.12296
          7653522
          24118380
          2debf31b-b1f4-41f2-96cb-57ebda8ddd0e
          © 2013 Japanese Cancer Association
          History
          : 07 June 2013
          : 09 September 2013
          : 19 September 2013
          Page count
          Pages: 8
          Funding
          Funded by: National Basic Research Program of China
          Award ID: 2011CB504303
          Funded by: Ministry of Science and Technology of China
          Award ID: 2011ZX09307‐001‐04
          Categories
          Original Article
          Original Articles
          Clinical Research
          Custom metadata
          2.0
          December 2013
          Converter:WILEY_ML3GV2_TO_JATSPMC version:5.9.3 mode:remove_FC converted:10.11.2020

          Comments

          Comment on this article