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Abstract
Glutamic acid decarboxylase (GAD, EC 4.1.1.15) catalyses the conversion of glutamate
to gamma-aminobutyric acid (GABA). The 65 kDa isoform, GAD65 is a potent autoantigen
in type 1 diabetes, whereas GAD67 is not. A hybrid cDNA was created by fusing a human
cDNA for amino acids 1-101 of GAD67 to a human cDNA for amino acids 96-585 of GAD65;
the recombinant (r) protein was expressed in yeast and was shown to have equivalent
immunoreactivity to mammalian brain GAD with diabetes sera. We here report on enzymatic
and molecular properties of rGAD67/65.