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      [Molecular analysis of natural infection of Lutzomyia longipalpis in an endemic area for visceral leishmaniasis in Brazil].

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          Abstract

          The main purpose of this study was to investigate natural infection by Leishmania chagasi in female sand flies in a visceral leishmaniasis (VL) focus on São Luís Island, Maranhão State, Brazil. Molecular analysis by polymerase chain reaction (PCR) was applied to determine the rate of natural infection of Lutzomyia longipalpis by L. chagasi in areas of old and recent human settlement on São Luís Island. Based on a sample of 800 female specimens captured from March to August 2005, the natural infection rate was 1.25% in an area of old settlement and 0.25% in two recently settled areas. Infection of L. longipalpis was detected in both areas, regardless of the number of reported human VL cases, indicating that other factors modulating infection in the wild need to be investigated. The results confirm PCR as a specific technique and an important tool for epidemiological surveillance.

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          Author and article information

          Journal
          Cad Saude Publica
          Cadernos de saude publica
          1678-4464
          0102-311X
          Dec 2010
          : 26
          : 12
          Affiliations
          [1 ] Universidade Federal do Piauí, Floriano, PI, Brasil. regianebiologia@yahoo.com.br
          Article
          S0102-311X2010001200019
          21243235
          2e96c474-5e50-4e2c-8a3c-ccf2f2eedc8f
          History

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