94
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Human cerebral organoids recapitulate gene expression programs of fetal neocortex development

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Cerebral organoids—3D cultures of human cerebral tissue derived from pluripotent stem cells—have emerged as models of human cortical development. However, the extent to which in vitro organoid systems recapitulate neural progenitor cell proliferation and neuronal differentiation programs observed in vivo remains unclear. Here we use single-cell RNA sequencing (scRNA-seq) to dissect and compare cell composition and progenitor-to-neuron lineage relationships in human cerebral organoids and fetal neocortex. Covariation network analysis using the fetal neocortex data reveals known and previously unidentified interactions among genes central to neural progenitor proliferation and neuronal differentiation. In the organoid, we detect diverse progenitors and differentiated cell types of neuronal and mesenchymal lineages and identify cells that derived from regions resembling the fetal neocortex. We find that these organoid cortical cells use gene expression programs remarkably similar to those of the fetal tissue to organize into cerebral cortex-like regions. Our comparison of in vivo and in vitro cortical single-cell transcriptomes illuminates the genetic features underlying human cortical development that can be studied in organoid cultures.

          Related collections

          Author and article information

          Journal
          Proceedings of the National Academy of Sciences
          Proc Natl Acad Sci USA
          Proceedings of the National Academy of Sciences
          0027-8424
          1091-6490
          December 22 2015
          December 22 2015
          December 22 2015
          December 07 2015
          : 112
          : 51
          : 15672-15677
          Article
          10.1073/pnas.1520760112
          4697386
          26644564
          2eaa2f5f-4b02-40b1-a00c-5d766fb4ce13
          © 2015

          Free to read

          http://www.pnas.org/preview_site/misc/userlicense.xhtml

          History

          Comments

          Comment on this article