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Size and shape of poly(ADP-ribose): Examination by gel filtration, gel electrophoresis and electron microscopy
April 1983
April 1983
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Abstract
Digestion of the poly(ADP-ribose) with snake venom phosphodiesterase yielded phosphoribosyl-AMP
and AMP in a ratio of 30 to 1, indicating the "chain length" of 30 if the poly(ADP-ribose)
is a linear molecule as previously supposed. Gel filtration and gel electrophoresis,
however, revealed a very heterogeneous size distribution, the average size being,
much longer than (ADP-ribose)30. Electron microscopy of poly(ADP-ribose) showed an
irregularly branched figure. Molecules with more than ten branched portions were observed.
These results are in agreement with the recent identification of the chemical structure
of the branched portion in poly(ADP-ribose).