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      Investigation of the Use of Positron Emission Tomography for Neuroreceptor Imaging in Rabbit Eyes

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          To determine whether positron emission tomography (PET) can be used for imaging of neuroreceptors in eyes of rabbits. PET imaging of dopamine D<sub>2</sub> receptor, dopamine transporter, serotonin<sub>1A</sub> receptor and sigma<sub>1</sub> receptor in the eyes and brain was performed using corresponding positron-emitting ligands in baseline, pretreatment and displacement conditions. The 4 radioligands outlined the eyes and brain in the baseline. Pretreatment resulted in a slight reduction (26–28%) in the uptake in the anterior segments of eyes. The binding of each radioligand in the iris-ciliary body and retina was confirmed by ex vivo autoradiography. However, the PET signal in the eyes was unexpectedly higher than the autoradiography signal. The identification of radioligand-neuroreceptor binding by PET in the rabbit eyes is not specific enough.

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          Most cited references 30

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          MicroPET: a high resolution PET scanner for imaging small animals

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            Localization of adenosine receptor messenger RNAs in the rat eye.

            Adenosine is present in all cells and body fluids and has been suggested to play several roles in the physiology of ocular tissues. The present study was undertaken to determine which types of adenosine receptor mRNAs are present in the rat eye, and where they are expressed. RNA or deoxyoligodeoxynucleotides complementary to rat adenosine receptor subtypes A1, A2A, A2B and A3 were used to generate 35S labeled antisense and sense probes. The probes were then used for in situ hybridization on 10 microm cryosections of the rat eye including the cornea, iris, ciliary body, lens, retina, choroid and sclera. A1, A2A and A2B receptor mRNAs were demonstrated in the ciliary processes. A1 receptor mRNA was also expressed in the ganglion cell layer of the retina. The retina also showed A2A receptor mRNA expression, which was most prominent in the inner nuclear layer and less prominent in the ganglion cell layer and outer nuclear layer. Weak A2A expression was found in the retinal pigment epithelium and choriocapillaris. No significant expression of A3 receptor mRNA was found in the rat eye. In conclusion, using in situ hybridization, we have demonstrated expression of mRNA for A1, A2A and A2B adenosine receptors in the rat eye. The expression patterns support specific roles for adenosine in the ciliary process and retina. Copyright 1997 Academic Press Limited.
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              A high resolution PET for animal studies


                Author and article information

                Ophthalmic Res
                Ophthalmic Research
                S. Karger AG
                October 2004
                26 November 2004
                : 36
                : 5
                : 255-263
                aPositron Medical Center, Tokyo Metropolitan Institute of Gerontology, and bDepartment of Ophthalmology and Visual Science, Tokyo Medical and Dental University, Tokyo, cNara Research and Development Center, Santen Pharmaceutical Company Ltd., Nara, and dM’s Sciences Company, Kobe, Japan
                81205 Ophthalmic Res 2004;36:255–263
                © 2004 S. Karger AG, Basel

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                Page count
                Figures: 4, Tables: 1, References: 44, Pages: 9
                Original Paper


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