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Virtual dissections through micro-CT scanning: a method for non-destructive genitalia ‘dissections’ of valuable Lepidoptera material : Virtual genitalia dissection

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Systematic Entomology

Wiley-Blackwell

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      Biological identifications through DNA barcodes.

      Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon 'barcodes'. We establish that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals. First, we demonstrate that COI profiles, derived from the low-density sampling of higher taxonomic categories, ordinarily assign newly analysed taxa to the appropriate phylum or order. Second, we demonstrate that species-level assignments can be obtained by creating comprehensive COI profiles. A model COI profile, based upon the analysis of a single individual from each of 200 closely allied species of lepidopterans, was 100% successful in correctly identifying subsequent specimens. When fully developed, a COI identification system will provide a reliable, cost-effective and accessible solution to the current problem of species identification. Its assembly will also generate important new insights into the diversification of life and the rules of molecular evolution.
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        A plea for DNA taxonomy

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          Myth of the molecule: DNA barcodes for species cannot replace morphology for identification and classification

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            Author and article information

            Journal
            Systematic Entomology
            Syst Entomol
            Wiley-Blackwell
            03076970
            July 2014
            July 2014
            : 39
            : 3
            : 606-618
            10.1111/syen.12067
            © 2014

            http://doi.wiley.com/10.1002/tdm_license_1.1

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            Self URI (article page): http://doi.wiley.com/10.1111/syen.12067

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