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      Marine urease with higher thermostability, pH and salinity tolerance from marine sponge-derived Penicillium steckii S4-4

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          Abstract

          Urease has a broad range of applications, however, the current studies on urease mainly focus on terrestrial plants or microbes. Thus, it is quite necessary to determine if marine-derived ureases have different characteristics from terrestrial origins since the finding of ureases with superior performance is of industrial interest. In this study, the marine urease produced by Penicillium steckii S4-4 derived from marine sponge Siphonochalina sp. was investigated. This marine urease exhibited a maximum specific activity of 1542.2 U mg protein −1. The molecular weight of the enzyme was 183 kDa and a single subunit of 47 kDa was detected, indicating that it was a tetramer. The N-terminal amino acid sequence of the urease was arranged as GPVLKKTKAAAV with greatest similarity to that from marine algae Ectocarpus siliculosus. This urease exhibited a K m of 7.3 mmol L −1 and a V max of 1.8 mmol urea min −1 mg protein −1. The optimum temperature, pH and salinity are 55 °C, 8.5 and 10%, respectively. This urease was stable and more than 80% of its maximum specific activity was detected after incubating at 25–60 °C for 30 min, pH 5.5–10.0 or 0–25% salinity for 6 h. Compared with the terrestrial urease from Jack bean, this marine urease shows higher thermostability, alkaline preference and salinity tolerance, which extends the potential application fields of urease to a great extent.

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          Author and article information

          Journal
          MLST
          Marine Life Science & Technology
          Springer (China )
          2096-6490
          2662-1746
          01 February 2021
          17 November 2020
          : 3
          : 1
          : 77-84
          Affiliations
          [1] 1Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
          Author notes
          *Corresponding author: Zhiyong Li, E-mail: zyli@ 123456sjtu.edu.cn
          Article
          s42995-020-00076-6
          10.1007/s42995-020-00076-6
          37073394
          2f1fb55e-57bf-4c75-ae8a-46ce46fc276d
          © 2020 Ocean University of China
          History
          : 07 March 2020
          : 16 September 2020
          Categories
          Research Papers

          Evolutionary Biology,Cell biology,Aquaculture & Fisheries,Ecology,Biotechnology,Life sciences
          Salinity tolerance, Penicillium steckii ,Alkali resistance,Marine urease,Thermostability

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