19
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro Translated title: Flúor não induz danos ao DNA em células de ovário de hamster chinês in vitro

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents.

          Translated abstract

          O flúor tem sido amplamente usado na Odontologia, pois é um agente profilático efetivo e específico contra a cárie dentária. Entretanto, o flúor em excesso pode representar perigos à saúde humana, especialmente por causar agressão ao material genético. Testes de genotoxicidade representam uma importante parte da pesquisa do câncer para a avaliação de risco de possíveis carcinógenos. Neste presente estudo, danos ao DNA associados à exposição ao flúor foram avaliados pelo teste de células individualizadas em gel de agarose (teste do cometa) in vitro. Células de ovário de hamster chinês foram expostas ao fluoreto de sódio (NaF) nas concentrações finais de 7 a 100 µg/ml, durante 3 h, a 37°C. Os resultados mostraram que o NaF não contribuiu para os danos no DNA em todas as concentrações testadas, conforme demonstrado pelas médias do momento da cauda e da intensidade da cauda dos cometas. Esses achados são clinicamente importantes, uma vez que representam uma importante contribuição para a correta avaliação do potencial risco à saúde associada à exposição aos agentes odontológicos.

          Related collections

          Most cited references26

          • Record: found
          • Abstract: not found
          • Article: not found

          Genotoxic effects of fluoride evaluated by sister-chromatid exchange

            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Genotoxic evaluation of chronic fluoride exposure: sister-chromatid exchange study

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Fluoride inhibition of protein and DNA synthesis in cells in vitro.

              Fluoride concentrations at and above 0.9 mM caused a progressive, concentration-related inhibition in the incorporation of both 14C-leucine and 3H-thymidine in LS cells incubated in medium with serum. The incorporation of leucine was more affected than that of thymidine. Lowering the pH enhanced the effect of fluoride on both. Removing serum from the incubation medium changed the effect of fluoride, particularly at low pH (7.0). Incorporation of leucine was then stimulated by low fluoride concentrations (0.5 and 0.9 mM), and the effect on thymidine incorporation was eradicated up to 1.3 mM-NaF. No differences were found in the pool and the specific activity of 14C-leucine in the fluoride exposed cells compared to control cells without fluoride (incubated at pH 7.4 in medium without serum). The cellular pool of 3H-thymidine decreased markedly during the incubation period, somewhat less in the fluoride exposed cells than in the control.
                Bookmark

                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                bor
                Brazilian Oral Research
                Braz. oral res.
                Sociedade Brasileira de Pesquisa Odontológica - SBPqO (São Paulo )
                1807-3107
                September 2004
                : 18
                : 3
                : 192-196
                Affiliations
                [1 ] Universidade Estadual Paulista Brazil
                [2 ] Universidade Estadual Paulista Brazil
                [3 ] Universidade Estadual Paulista Brazil
                Article
                S1806-83242004000300003
                10.1590/S1806-83242004000300003
                301480cd-9ac6-4d89-a0c2-38cad059a3ee

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1806-8324&lng=en
                Categories
                DENTISTRY, ORAL SURGERY & MEDICINE

                Dentistry
                Sodium fluoride,Comet assay,Mutagenicity tests,Fluoreto de sódio,Ensaio em cometa,Testes de mutagenicidade

                Comments

                Comment on this article