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Abstract
Pressurised water extraction (PWE) of polysaccharides from blackcurrant fruits was
investigated using a response surface methodology (RSM). The optimal conditions for
PWE were: time 51min, pressure 1.6MPa, and temperature 52°C. Under these conditions,
the experimental yield of Ribes nigrum L. polysaccharides (RNLP) was 11.68±0.12%,
which closely agreed with the predicted value (11.77%). After preliminary purification
with D4006 macroporous resin, RNLP I was obtained and its chemical characterisation
was undertaken by GC, HPLC, and IR spectroscopy. RNLP I was composed of rhamnose,
arabinose, xylose, mannose, galactose, and glucose with a molar ratio of 2.89:14.82:1.02:1.00:2.53:6.39
and its molecular weight was 1.49×10(4)kDa. The antioxidant activity of RNLP I was
evaluated by free radical scavenging assays and a reducing power assay in vitro. RNLP
I showed strong DPPH and superoxide radical scavenging activities and reducing power.