Genetic linkage maps of &apos;Pêra&apos; sweet orange and &apos;Cravo&apos; mandarin with RAPD markers <span class="so-article-trans-title" dir="auto"> Translated title: Mapas genéticos de ligação de laranja &apos;Pêra&apos; e tangerina &apos;Cravo&apos; utilizando marcadores RAPD </span>

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Abstract

The objective of this work was to construct linkage maps of 'Pêra' sweet orange [Citrus sinensis (L.) Osbeck] and 'Cravo' mandarin (Citrus reticulata Blanco) using RAPD markers and the pseudo-testcross strategy. The parents were chosen according to the resistance/susceptibility to citrus variegate chlorosis (CVC). The segregation of 176 markers was analyzed in 94 progeny of F1 hybrids, which were obtained from controlled crossings. The linkage map of 'Pêra' sweet orange had 117 markers defined by 12 linkage groups, which spanned 612.1 cM. Only six markers could not be linked to the linkage group and 48.7% of the markers showed segregation distortion. The linkage map of 'Cravo' mandarin had 51 markers defined by 12 linkage groups, which spanned 353.3 cM. Only two markers did not link to the groups and 15.7% showed segregation distortion. The construction of linkage maps is relevant to future mapping studies of the inheritance of CVC, citrus canker and leprosis.

Translated abstract

O objetivo deste trabalho foi elaborar mapas de ligação de laranja 'Pêra' [Citrus sinensis (L.) Osbeck] e tangerina 'Cravo' (Citrus reticulata Blanco) utilizando marcadores RAPD e a estratégia pseudo-testcross. Os parentais foram escolhidos tomando-se por base o nível de resistência/suscetibilidade à clorose variegada dos citros (CVC). A segregação de 176 marcadores foi analisada em uma progênie de 94 híbridos F1, obtidos por meio de cruzamentos controlados. O mapa de ligação de laranja 'Pêra' apresentou 117 marcadores distribuídos em 12 grupos de ligação, totalizando 612,1 cM. Apenas seis marcadores não se ligaram a algum dos grupos de ligação e 48,7% deles apresentaram segregação distorcida. O mapa de ligação de tangerina 'Cravo' apresentou 51 marcadores distribuídos em 12 grupos de ligação, totalizando 353,3 cM. Apenas dois marcadores apresentaram-se desligados e 15,7% deles apresentaram segregação distorcida. A elaboração de mapas de ligação dessas cultivares é relevante para futuros estudos da herança da resistência à CVC, cancro cítrico e leprose.

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Most cited references 24

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Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers.

D Grattapaglia, R. Sederoff (1994)

We have used a "two-way pseudo-testcross" mapping strategy in combination with the random amplified polymorphic DNA (RAPD) assay to construct two moderate density genetic linkage maps for species of Eucalyptus. In the cross between two heterozygous individuals many single-dose RAPD markers will be heterozygous in one parent, null in the other and therefore segregate 1:1 in their F1 progeny following a testcross configuration. Meiosis and gametic segregation in each individual can be directly and efficiently analyzed using RAPD markers. We screened 305 primers of arbitrary sequence, and selected 151 to amplify a total of 558 markers. These markers were grouped at LOD 5.0, theta = 0.25, resulting in the maternal Eucalyptus grandis map having a total of 240 markers into 14 linkage groups (1552 cM) and the paternal Eucalyptus urophylla map with 251 markers in 11 linkage groups (1101 cM) (n = 11 in Eucalyptus). Framework maps ordered with a likelihood support > or = 1000:1 were assembled covering 95% of the estimated genome size in both individuals. Characterization of genome complexity of a sample of 48 mapped random amplified polymorphic DNA (RAPD) markers indicate that 53% amplify from low copy regions. These are the first reported high coverage linkage maps for any species of Eucalyptus and among the first for any hardwood tree species. We propose the combined use of RAPD markers and the pseudo-testcross configuration as a general strategy for the construction of single individual genetic linkage maps in outbred forest trees as well as in any highly heterozygous sexually reproducing living organisms. A survey of the occurrence of RAPD markers in different individuals suggests that the pseudo-testcross/RAPD mapping strategy should also be efficient at the intraspecific level and increasingly so with crosses of genetically divergent individuals. The ability to quickly construct single-tree genetic linkage maps in any forest species opens the way for a shift from the paradigm of a species index map to the heterodox proposal of constructing several maps for individual trees of a population, therefore mitigating the problem of linkage equilibrium between marker and trait loci for the application of marker assisted strategies in tree breeding.

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Eric S. Lander, Philip Green, Jeff Abrahamson … (1987)

With the advent of RFLPs, genetic linkage maps are now being assembled for a number of organisms including both inbred experimental populations such as maize and outbred natural populations such as humans. Accurate construction of such genetic maps requires multipoint linkage analysis of particular types of pedigrees. We describe here a computer package, called MAPMAKER, designed specifically for this purpose. The program uses an efficient algorithm that allows simultaneous multipoint analysis of any number of loci. MAPMAKER also includes an interactive command language that makes it easy for a geneticist to explore linkage data. MAPMAKER has been applied to the construction of linkage maps in a number of organisms, including the human and several plants, and we outline the mapping strategies that have been used.

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Phylogenetic relationships within the genus Citrus ( Rutaceae ) and related genera as revealed by RFLP and RAPD analysis

C. T. Federici, D. Q. Fang, R. W. Scora … (1998)

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Author and article information

Journal

Journal ID (publisher-id): pab

Title: Pesquisa Agropecuária Brasileira

Abbreviated Title: Pesq. agropec. bras.

Publisher: Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira (Brasília, DF, Brazil )

ISSN (Print): 0100-204X

ISSN (Electronic): 1678-3921

Publication date (Print and electronic): February 2004

Volume: 39

Issue: 2

Pages: 159-165

Affiliations

[02] Cordeirópolis SP orgnameCentro Apta Citros Sylvio Moreira

[01] Pelotas RS orgnameEmbrapa Clima Temperado rpedroso@ 123456cpact.embrapa.br

Article

Publisher ID: S0100-204X2004000200009 Publisher ID: S0100-204X(04)03900209

SO-VID: 30499720-e74f-42a1-9b7e-1b93a86f2bb7

License:

This work is licensed under a Creative Commons Attribution 4.0 International License.

History

Date accepted : 26 November 2003

Date received : 17 June 2003

Page count

Figures: 0, Tables: 0, Equations: 0, References: 26, Pages: 7

Product

SciELO Brazil

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers Translated title: Mapas genéticos de ligação de laranja 'Pêra' e tangerina 'Cravo' utilizando marcadores RAPD

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