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      Enhancing biomass and lipid productivity of a green microalga Parachlorella kessleri for biodiesel production using rapid mutation of atmospheric and room temperature plasma

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          Abstract

          Background

          Microalgae, with their high adaptability to various stress conditions and rapid growth, are considered excellent biomass resources for lipid production and biodiesel feedstocks. However, lipid yield and productivity of the natural strains are common bottlenecks in their large-scale use for lipid production, which can be overcome by evolving new strains using conventional and advanced mutagenic techniques. It is challenging to generate microalgae strains capable of high lipid synthesis through natural selection. As a result, random mutagenesis is currently considered a viable option in many scenarios. The objective of this study was to explore atmospheric and room temperature plasma (ARTP) as a random mutagenesis technique to obtain high lipid-accumulating mutants of a green microalga for improved biodiesel production.

          Results

          A green microalgal species was isolated from the Chinese Yellow Sea and identified as Parachlorella kessleri (OM758328). The isolated microalga was subsequently mutated by ARTP to obtain high lipid-accumulating mutants. Based on the growth rate and lipid content, 5 mutants (named M1, M2, M4, M5, and M8) were selected from 15 pre-selected mutants. These five mutants varied in their growth rate from 0.33 to 0.68 day −1, with the lipid content varying between 0.25 g/L in M2 to 0.30 g/L in M8 at 10th day of cultivation. Among the mutants, M8 showed the maximum biomass productivity (0.046 g/L/day) and lipid productivity (20.19 mg/L/day), which were 75% and 44% higher than the wild strain, respectively. The triglyceride (TAG) content of M8 was found to be 0.56 g/L at 16th day of cultivation, which was 1.77-fold higher than that of the wild strain. Furthermore, M8 had the highest saturated fatty acids (C16-18) with the lowermost polyunsaturated fatty acid content, which are favorable properties of a biodiesel feedstock according to international standards.

          Conclusion

          The mutant strain of P. kessleri developed by the ARTP technique exhibited significant improvements in biomass productivity, lipid content, and biodiesel quality. Therefore, the biomass of this mutant microalga could be a potential feedstock for biodiesel production.

          Supplementary Information

          The online version contains supplementary material available at 10.1186/s13068-022-02220-z.

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          Most cited references67

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                Author and article information

                Contributors
                mostafa_elshobary@science.tanta.edu.eg
                Journal
                Biotechnol Biofuels Bioprod
                Biotechnol Biofuels Bioprod
                Biotechnology for Biofuels and Bioproducts
                BioMed Central (London )
                2731-3654
                13 November 2022
                13 November 2022
                2022
                : 15
                : 122
                Affiliations
                [1 ]GRID grid.412258.8, ISNI 0000 0000 9477 7793, Department of Botany, Faculty of Science, , Tanta University, ; Tanta, 31527 Egypt
                [2 ]GRID grid.440785.a, ISNI 0000 0001 0743 511X, School of Food and Biological Engineering, , Jiangsu University, ; Zhenjiang, 212013 Jiangsu China
                Author information
                http://orcid.org/0000-0002-2131-0679
                Article
                2220
                10.1186/s13068-022-02220-z
                9655907
                36372889
                3079b461-b9b2-48cc-9143-b3000e98f66a
                © The Author(s) 2022

                Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

                History
                : 12 June 2022
                : 23 October 2022
                Categories
                Research
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                © The Author(s) 2022

                atmospheric and room temperature plasma,parachlorella kessleri,lipid production,biodiesel quality,fatty acid,triglyceride

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