23
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Effects of insect cadavers infected by Heterorhabditis bacteriophora and Steinernema diaprepesi on Meloidogyne incognita parasitism in pepper and summer squash plants Translated title: Efecto de insectos cadáveres infectados por Heterorhabditis bacteriophora y Steinernema diaprepesi sobre el parasitismo de Meloidogyne incognita en plantas de pimiento y zapallito redondo de tronco

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The effects of insect cadavers infected with three isolates of Heterorhabditis bacteriophora and one isolate of Steinernema diaprepesi on a population of Meloidogyne incognita in pepper (Capsicum annuum) and summer squash (Cucurbita maxima) were evaluated in greenhouse experiments carried out in Santa Fe (Argentina). Insect cadavers were obtained for the experiments from last instar larvae of Galleria mellonella and Tenebrio molitor that had been infected with entomopathogenic nematodes. Two six-day-old insect cadavers per pot were placed below the soil surface, and the soil was inoculated with 100 second-stage juveniles ofM incognita. Sixty days after inoculation, the following parameters were recorded for each plant: number of leaves; dry weight of aerial parts; numbers of galls, egg masses and eggs; and numbers of galls, egg masses and eggs g-1 of root fresh matter. In pepper, the only variable affected by the infected cadavers with respect to control was the number of eggs in the treatment involving T. molitor cadavers infected with the H. bacteriophora isolate Rama Caída. In summer squash, several treatments using infected cadavers resulted in a decrease in the numbers of galls and egg masses. Only the treatment involving G. mellonella cadavers infected with the H. bacteriophora isolate Rama Caída proved to be efficient in reducing the number of M. incognita eggs. Our results indicated that the application of insect cadavers infected with the entomopathogenic nematodes studied might reduce M. incognita damage in pepper and summer squash plants.

          Translated abstract

          El efecto de cadáveres de insectos infectados con tres aislados de Heterorhabditis bacteriophora y un aislado de Steinernema diaprepesi sobre una población de Meloidogyne incognita en plantas de pimiento (Capsicum annuum) y zapallito redondo de tronco (Cucurbita maxima) fue evaluado en experiencias de invernadero conducidas en Santa Fe (Argentina). Los cadáveres de insectos necesarios para la experiencia se obtuvieron infectando larvas de último estadio de Galleria mellonella y Tenebrio monitor con nematodos entomopatógenos. Dos cadáveres de seis días de infección se colocaron debajo de la superficie del suelo de macetas que fueron inoculadas con 100 juveniles de segundo estadio de M. incognita. A los sesenta días se registraron los siguientes parámetros en cada planta: número de hojas, peso seco de la parte aérea, número de agallas, masas de huevos y huevos, y número de agallas, masas de huevos y huevos g-1 de materia fresca radical. En pimiento, la única variable afectada por los cadáveres en relación al testigo fue el número de huevos de M. incognita en el tratamiento de cadáveres de T. molitor infectados con H. bacteriophora aislado Rama Caída. En zapallito redondo de tronco, varios tratamientos provocaron una disminución en el número de agallas y masas de huevos. Sólo la aplicación de cadáveres de G. mellonella infectados con H. bacteriophora aislado Rama Caída demostró ser eficiente en reducir el número de huevos de M. incognita. Nuestros resultados indicaron que la aplicación de cadáveres de insectos infectados con los nematodos entomopatógenos estudiados podría reducir los daños causados por M. incognita en plantas de pimiento y zapallito redondo de tronco.

          Related collections

          Most cited references43

          • Record: found
          • Abstract: found
          • Article: not found

          Survey of crop losses in response to phytoparasitic nematodes in the United States for 1994.

          Previous reports of crop losses to plant-parasitic nematodes have relied on published results of survey data based on certain commodities, including tobacco, peanuts, cotton, and soybean. Reports on crop-loss assessment by land-grant universities and many commodity groups generally are no longer available, with the exception of the University of Georgia, the Beltwide Cotton Conference, and selected groups concerned with soybean. The Society of Nematologists Extension Committee contacted extension personnel in 49 U.S. states for information on estimated crop losses caused by plant-parasitic nematodes in major crops for the year 1994. Included in this paper are survey results from 35 states on various crops including corn, cotton, soybean, peanut, wheat, rice, sugarcane, sorghum, tobacco, numerous vegetable crops, fruit and nut crops, and golf greens. The data are reported systematically by state and include the estimated loss, hectarage of production, source of information, nematode species or taxon when available, and crop value. The major genera of phytoparasitic nematodes reported to cause crop losses were Heterodera, Hoplolaimus, Meloidogyne, Pratylenchus, Rotylenchulus, and Xiphinema.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Xenorhabdus gen. nov., a Genus of Entomopathogenic, Nematophilic Bacteria of the Family Enterobacteriaceae

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Entomopathogenic nematode production and application technology.

              Production and application technology is critical for the success of entomopathogenic nematodes (EPNs) in biological control. Production approaches include in vivo, and in vitro methods (solid or liquid fermentation). For laboratory use and small scale field experiments, in vivo production of EPNs appears to be the appropriate method. In vivo production is also appropriate for niche markets and small growers where a lack of capital, scientific expertise or infrastructure cannot justify large investments into in vitro culture technology. In vitro technology is used when large scale production is needed at reasonable quality and cost. Infective juveniles of entomopathogenic nematodes are usually applied using various spray equipment and standard irrigation systems. Enhanced efficacy in EPN applications can be facilitated through improved delivery mechanisms (e.g., cadaver application) or optimization of spray equipment. Substantial progress has been made in recent years in developing EPN formulations, particularly for above ground applications, e.g., mixing EPNs with surfactants or polymers or with sprayable gels. Bait formulations and insect host cadavers can enhance EPN persistence and reduce the quantity of nematodes required per unit area. This review provides a summary and analysis of factors that affect production and application of EPNs and offers insights for their future in biological insect suppression.
                Bookmark

                Author and article information

                Journal
                ciagr
                Ciencia e investigación agraria
                Cienc. Inv. Agr.
                Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal (Santiago, , Chile )
                0718-1620
                April 2013
                : 40
                : 1
                : 109-118
                Affiliations
                [03] Buenos Aires orgnameConsejo Nacional de Investigaciones Científicas y Técnicas Argentina
                [04] Córdoba orgnameLaboratorio de Biología Molecular Argentina
                [01] Santa Fe orgnameUniversidad Nacional del Litoral orgdiv1Facultad de Ciencias Agrarias Argentina edelvalle@ 123456fca.unl.edu.ar
                [02] Córdoba orgnameJardín Zoológico orgdiv1Centro de Zoología Aplicada orgdiv2Laboratorio de Nematología Argentina
                Article
                S0718-16202013000100009 S0718-1620(13)04000100009
                10.4067/S0718-16202013000100009
                3085e705-29e0-4ec9-a0ce-275ba7a37005

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 07 July 2012
                : 29 January 2013
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 45, Pages: 10
                Product

                SciELO Chile

                Categories
                RESEARCH PAPERS

                Biological control,nematodos entomopatógenos,control biológico,Cadáveres de insectos infectados,insect host cadavers,entomopathogenic nematodes

                Comments

                Comment on this article