Magnetite (Fe3O4) forms the basis of most dispersions studied in the field of magnetic fluids and magnetic colloids. Despite extensive theory and simulations on chain formation in dipolar fluids in zero field, such structures have not yet been imaged in laboratory-made magnetite dispersions. Here, we present the first direct observation of dipolar chain formation in zero field in a ferrofluid containing the largest synthetic single-domain magnetite particles studied so far. To our knowledge, this is the only ferrofluid system available at present that allows quantifying chain length and ring-size distributions of dipolar structures as a function of concentration and particle size.