For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
193
views
20
references
 Top references
cited by
304
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      5,239
      similar
       All similar
      • Record: found
      • Abstract: not found
      • Article: not found

      Fluorogenic probes for live-cell imaging of the cytoskeleton

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells.

          Related collections

          Most cited references20

          • Record: found
          • Abstract: not found
          • Article: not found

          Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. SHORT COMMUNICATION

          M. Gustafsson (2000)
          Article 0 comments Cited 380 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.

            Gražvydas Lukinavičius, Keitaro Umezawa, Nicolas Olivier … (2013)
            The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.
            Article 0 comments Cited 252 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Aurora B-mediated abscission checkpoint protects against tetraploidization.

              Patrick Steigemann, Claudia Wurzenberger, Michael Schmitz … (2009)
              Genomic abnormalities are often seen in tumor cells, and tetraploidization, which results from failures during cytokinesis, is presumed to be an early step in cancer formation. Here, we report a cell division control mechanism that prevents tetraploidization in human cells with perturbed chromosome segregation. First, we found that Aurora B inactivation promotes completion of cytokinesis by abscission. Chromosome bridges sustained Aurora B activity to posttelophase stages and thereby delayed abscission at stabilized intercellular canals. This was essential to suppress tetraploidization by furrow regression in a pathway further involving the phosphorylation of mitotic kinesin-like protein 1 (Mklp1). We propose that Aurora B is part of a sensor that responds to unsegregated chromatin at the cleavage site. Our study provides evidence that in human cells abscission is coordinated with the completion of chromosome segregation to protect against tetraploidization by furrow regression.
              Article 0 comments Cited 223 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Title: Nature Methods
                Abbreviated Title: Nat Methods
                Publisher: Springer Science and Business Media LLC
                ISSN (Print): 1548-7091
                ISSN (Electronic): 1548-7105
                Publication date Created: July 2014
                Publication date (Electronic): May 25 2014
                Publication date (Print): July 2014
                Volume: 11
                Issue: 7
                Pages: 731-733
                Article
                DOI: 10.1038/nmeth.2972
                PubMed ID: 24859753
                SO-VID: 3129e327-19a7-4a4e-80a6-62b6e7684bd2
                Copyright © © 2014
                License:

                http://www.springer.com/tdm

                History

                Data availability:

                Comments

                Comment on this article

                scite_

                Similar content5,239

                See all similar

                Cited by298

                See all cited by

                Most referenced authors459

                See all reference authors