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      Lung albumin accumulation is spatially heterogeneous but not correlated with regional pulmonary perfusion

      1 , 1 , 2
      Journal of Applied Physiology
      American Physiological Society

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          Preparation of iodine-131 labelled human growth hormone of high specific activity.

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            FRACTURE FACES OF ZONULAE OCCLUDENTES FROM "TIGHT" AND "LEAKY" EPITHELIA

            Epithelia vary with respect to transepithelial permeability. In those that are considered "leaky", a large fraction of the passive transepithelial flux appears to follow the paracellular route, passing across the zonulae occludentes and moving down the intercellular clefts. In "tight" epithelia, the resistance of the paracellular pathway to passive flux is greatly increased. To see whether differences in the morphology of the zonula occludens could contribute to this variability in leakiness among epithelia, replicas of zonulae occludentes in freeze-fractured material from a variety of tight and leaky epithelia were examined. The junctions appear as a branching and anastomosing network of strands or grooves on the A and B membrane fracture faces, respectively. It was found that the zonula occludens from a "very leaky" epithelium, the proximal convoluted tubule of the mouse kidney, is extremely shallow in the apical-basal direction, consisting in most places of only one junctional strand. In contrast, the "very tight" frog urinary bladder exhibits a zonula occludens that is relatively deep (>0.5 µm) in the apical-basal direction, and consists of five or more interconnected junctional strands interposed between luminal and lateral membrane surfaces. Epithelia of intermediate permeabilities exhibited junctions with intermediate or variable morphology. Toad urinary bladder, mouse stomach, jejunum, and distal tubule, rabbit gallbladder, and Necturus kidney and gallbladder were also examined, and the morphological data from these epithelia were compared to physiological data from the literature.
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              Body Position Changes Redistribute Lung Computed-Tomographic Density in Patients with Acute Respiratory Failure

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                Author and article information

                Journal
                Journal of Applied Physiology
                Journal of Applied Physiology
                American Physiological Society
                8750-7587
                1522-1601
                January 2002
                January 2002
                : 92
                : 1
                : 279-287
                Affiliations
                [1 ]Departments of Medicine and
                [2 ]Physiology and Biophysics, University of Washington, Seattle, Washington 98195
                Article
                10.1152/japplphysiol.00353.2001
                3149a7ee-e625-4d8b-a605-fb28da527dfe
                © 2002
                History

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