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      Decreased Glomerular Proteinase Activity in the Streptozotocin Diabetic Rat

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          Decreased glomerular proteinase activity may contribute to matrix accumulation in diabetes. Male Sprague-Dawley rats were rendered diabetic by injection of streptozotocin (STZ) 65 mg/kg i.v.; age-matched, sham-injected rats served as controls. Glomeruli from diabetic rats 1 month after STZ injection demonstrated significant decreases in collagenase and cathepsin B activities compared to control glomeruli. Treatment with insulin resulted in a slight (but not significant) increase in collagenase activity and normalized cathepsin B activity. We conclude that decreased glomerular collagenase and cathepsin B activities are present in STZ diabetes. These alterations may contribute to mesangial matrix accumulation.

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          Glomerulopathy in rats with streptozotocin diabetes. Accumulation of glomerular basement membrane analogous to human diabetic nephropathy

           CV Klein,  MP Cohen (1979)
          Glomeruli from streptozotocin-diabetic and age-matched nondiabetic rats were quantitatively isolated by a differential sieving technique. The insoluble glomerular basement membranes were purified following sonic disruption in the presence of proteolytic inhibitors. The yield of glomeruli and of glomerular basement membrane relative to the amount of renal cortex and the body weight of the animals, as well as the calculated amount of basement membrane per glomerulus, were all significantly greater in diabetic rats when compared to non-diabetic controls. Glomerular basement membranes from normal and diabetic rats were solubilized by reduction and denaturation in the presence of SDS and subjected to agarose gel analysis. About 65% of both normal and diabetic basement membrane was solubilized by this procedure, and the elution profiles of non-diabetic and diabetic preparations were similar. These results suggest that rat renal basement membrane is qualitatively similar but quantitatively increased in streptozotocin- diabetes. Since glomerular enlargement and accumulation of basement membrane are characteristic of human diabetic nephropathy, the findings also suggest that the streptozotocin-diabetic rat is an appropriate animal model for studies relating to the pathogenesis of this complication of diabetes.
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            Glomerular metalloprotease activity in streptozotocin-treated rats and in spontaneously diabetic rats (BB/DP)


              Author and article information

              Am J Nephrol
              American Journal of Nephrology
              S. Karger AG
              June 1999
              18 June 1999
              : 19
              : 3
              : 441-446
              Department of Medicine, Veterans Affairs Hospital, Hines, Ill., and Loyola University Chicago Stritch School of Medicine, Maywood, Ill., USA
              13492 Am J Nephrol 1999;19:441–446
              © 1999 S. Karger AG, Basel

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              Page count
              Figures: 3, Tables: 1, References: 32, Pages: 6
              Self URI (application/pdf):
              Laboratory Investigation

              Cardiovascular Medicine, Nephrology

              Extracellular matrix, Collagenase, Diabetes, Mesangium, Streptozotocin


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