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      Erythrocyte Insulin Receptors in Chronic Renal Failure

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          Abstract

          Chronic renal failure is associated with altered insulin sensitivity of unclear etiology. To investigate the effect of renal failure on insulin binding, we studied insulin binding in mature erythrocytes from 5 undialyzed and 12 chronically dialyzed patients with renal failure. The cell suspension of the isolated and purified human erythrocytes (3.52 × 10<sup>9</sup>/ml) was incubated with 100 pg of <sup>125</sup>I-insulin and a range of unlabeled insulin concentrations (pH 8.0, 15°C for 3.5 h). A maximum of 7.9 ± 1.6 and 14.1 ± 3.5 (mean ± SD) specific percent of <sup>125</sup>I-insulin was bound by the undialyzed and dialyzed patients respectively, as compared to 10.1 ± 1.4 (mean ± SD) maximum specific percent of insulin bound by the normal subjects. Scatchard plots from the normal, undialyzed and dialyzed subjects were curvilinear, indicating negatively cooperative insulin receptor site-site interactions. From the DeMeyts1 analyses of the Scatchard plots, it was determined that the unoccupied receptor site affinity constant, K<sub>e</sub>, for the normal subjects was 0.5 × 108 M<sup>-1</sup> whereas that for the undialyzed and the dialyzed patients was 0.69 × 108 M<sup>-1</sup>. The normal subjects had 410 receptor sites per erythrocyte; however, the undialyzed patients had 215 sites per cell and the dialyzed patients had a receptor number similar to that of the normal subjects. The undialyzed patients had 48% fewer receptor sites than the dialyzed patients and the normal subjects. The undialyzed patients comparing with the normal subjects thus, showed a reduction in insulin binding and in the number of receptor sites with increased affinity. Chronic dialysis caused an increase of insulin binding and of receptor number when compared with the undialyzed patients. In the undialyzed patients with renal failure, the defect in receptor number may be associated with a defect in tissue response to insulin. This defect in insulin receptor number was removed with chronic dialysis. Thus, chronic dialysis may improve insulin sensitivity at the cellular level.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1981
          1981
          02 December 2008
          : 28
          : 1
          : 4-10
          Affiliations
          Endocrine and Renal Sections, Department of Medicine, College of Medicine, Howard University, Washington, D.C., USA
          Article
          182086 Nephron 1981;28:4–10
          10.1159/000182086
          7266727
          © 1981 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 7
          Categories
          Original Paper

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