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      In vitro Plasma Inactivation of Thyrotropin Releasing Factor (TRF) and Related Peptides. Its Inhibition by Various Means and by the Synthetic Dipeptide PCA-His-OME

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          Abstract

          Incubation in vitro of natural (ovine) or synthetic TRF (PCA-His-Pro-NH<sub>2</sub>) with rat blood, plasma, or reconstituted lyophilized serum destroys the biological activity. Inactivation of TRF by normal plasma takes place at 0 °C but at a slower rate than at 37 °C. Plasma obtained from rats several weeks after hypophysectomy and/ or hypophysectomy + thyroidectomy inactivates TRF. TRF activity is not recovered from TRF incubated in plasma when the incubate is treated with NaCl (4 m), ethanol (80% v/v) or HCl (to pH 1). Plasma inactivation of TRF is completely prevented by pretreatment of the plasma with ethanol (80% v/v); heating of plasma at 65 °C for 15 min also destroys the ability of plasma to inactivate TRF. The data reported are consistent with the hypothesis that plasma contains an enzyme (s) which destroys the activity of TRF. Plasma was also found to destroy the biological activity of PCA-His-Pro-OMe and PCA-His-Pro-OH (2 synthetic tripeptides with TRF activity). The biological activity of PCA-His-Pro-OH is destroyed in vitro at a slower rate by plasma than that of PCA-His-Pro-NH<sub>2</sub> or natural TRF. Addition of the dipeptide analogue of TRF, PCA-His-OMe, prevents in vitro the inactivation of TRF by plasma.

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          Author and article information

          Journal
          HRE
          Horm Res Paediatr
          10.1159/issn.1663-2818
          Hormone Research in Paediatrics
          S. Karger AG
          1663-2818
          1663-2826
          1971
          1971
          21 November 2008
          : 2
          : 4
          : 193-203
          Affiliations
          The Salk Institute for Biological Studies, San Diego, Calif.
          Article
          178234 Hormones 1971;2:193–203
          10.1159/000178234
          31bfd0a9-7a2c-45bb-b47d-5f2c1aedf8ca
          © 1971 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          Page count
          Pages: 11
          Categories
          Paper

          Endocrinology & Diabetes,Neurology,Nutrition & Dietetics,Sexual medicine,Internal medicine,Pharmacology & Pharmaceutical medicine
          Dipeptide,Inactivation,Inhibition,TRF,Polypeptides,TRF-analogues,Enzyme,Plasma

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