167
views
0
recommends
+1 Recommend
1 collections
    10
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Hypothalamic-Pituitary-Gonadal (HPG) Axis and Transcriptional Regulatory Elements Regulate piwil2 Gene Expression During Gametogenesis and Gonadal Development in Japanese Flounder ( Paralichthys olivaceus)

      research-article

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The P-element induced wimpy testis (Piwi) proteins, which are associated with PIWI-interacting RNAs (piRNAs), play important roles in meiosis, germ cell division, and germline maintenance. In this study, we identified and characterized the Paralichthys olivaceus piwil2 gene, a constituent factor of the piRNA pathways involved in the biogenesis of reproductive development. The biological analysis indicated that piwil2, which contains PAZ and PIWI domains, was highly conserved between teleosts and tetrapods. The piwil2 distribution profile in different tissues confirmed a sexually dimorphic expression pattern, with a higher expression level in testis. In situ hybridization demonstrated that piwil2 was expressed in the oogonia and oocytes of the ovaries as well as in the Sertoli cells and spermatocytes of the testes. Gene piwil2 showed a maternally inherited expression pattern during embryonic development, and was highly expressed during the early embryonic development. Different luciferase reporters were constructed to determine the transcriptional regulatory mechanisms of piwil2. The piwil2 core promoter region was located at −360 bp to −60 bp. Furthermore, some representative sex hormones, including human chorionic gonadotropin, 17α-methyltestosterone, and estradiol-17β had distinct regulatory effects on piwil2. In a summery, these results indicate that piwil2, regulated by sex hormones and transcriptional elements, has vital functions in the reproductive cycle and gonadal development.

          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          15 November 2020
          01 December 2020
          : 19
          : 6
          : 1378-1388
          Affiliations
          [1] 1Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao 266003, China
          [2] 2College of Life Sciences, Yantai University, Yantai 264005, China
          Author notes
          *Corresponding author: YU Haiyang, Tel: 0086-532-82031986; E-mail: yuhaiyang@ 123456ouc.edu.cn
          Article
          s11802-020-4442-0
          10.1007/s11802-020-4442-0
          31ca40ec-7317-4db4-8d01-34fca9233a17
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

          History
          : 07 January 2020
          : 03 March 2020
          : 19 June 2020

          Earth & Environmental sciences,Geology & Mineralogy,Oceanography & Hydrology,Aquaculture & Fisheries,Ecology,Animal science & Zoology
          Paralichthys olivaceus ,HPG axis,gametogenesis and gonadal development, piwil2 ,transcriptional regulatory elements

          Comments

          Comment on this article