The complete mitochondrial genome of long-tailed whiskered bat,  Myotis frater  (Myotis, Vespertilionidae)

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Abstract

Here, we report the complete mitogenome of Myotis frater with the GenBank accession number MH177276 as a first step to elucidate genetic characteristics of this species. Its mitogenome was 17,089 bp long and consisted of 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes, and a control region. The gene order and composition of M. frater was similar to that of most other vertebrates. The base composition of the 13 PCG in descending order was A (33.8%), C (22.7%), T (30.4%), and G (13.1%), with an AT content of 64.2%. Four overlapping regions in ATP8/ ATP6, ATP6/COX3, ND4L/ ND4, and ND5/ ND6, among the 13 PCGs were found. The 935 bp long control region is located between tRNA-Pro and tRNA-Phe with 4 ATTACATAATACATTATATGTATAATCGTACATTAAATTAACTCCCACATGAATATTAAGCATGTCCATACTAATATTAAT-repeat at 5′ region and 45 ACGCAT-repeat at 3′ terminus. Phylogenetic analysis suggested that M. frater is most closely related to M. bechsteinii (KX757757), it was supported by 100% bootstrap under both ML and NJ tree.

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Most cited references 4

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tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.

Todd Lowe, Sean R. Eddy (1997)

We describe a program, tRNAscan-SE, which identifies 99-100% of transfer RNA genes in DNA sequence while giving less than one false positive per 15 gigabases. Two previously described tRNA detection programs are used as fast, first-pass prefilters to identify candidate tRNAs, which are then analyzed by a highly selective tRNA covariance model. This work represents a practical application of RNA covariance models, which are general, probabilistic secondary structure profiles based on stochastic context-free grammars. tRNAscan-SE searches at approximately 30 000 bp/s. Additional extensions to tRNAscan-SE detect unusual tRNA homologues such as selenocysteine tRNAs, tRNA-derived repetitive elements and tRNA pseudogenes.

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Sequence evolution of mitochondrial tRNA genes and deep-branch animal phylogenetics.

M Nishida, Y Kumazawa (1993)

Mitochondrial DNA sequences are often used to construct molecular phylogenetic trees among closely related animals. In order to examine the usefulness of mtDNA sequences for deep-branch phylogenetics, genes in previously reported mtDNA sequences were analyzed among several animals that diverged 20-600 million years ago. Unambiguous alignment was achieved for stem-forming regions of mitochondrial tRNA genes by virtue of their conservative secondary structures. Sequences derived from stem parts of the mitochondrial tRNA genes appeared to accumulate much variation linearly for a long period of time: nearly 100 Myr for transition differences and more than 350 Myr for transversion differences. This characteristic could be attributed, in part, to the structural variability of mitochondrial tRNAs, which have fewer restrictions on their tertiary structure than do nonmitochondrial tRNAs. The tRNA sequence data served to reconstruct a well-established phylogeny of the animals with 100% bootstrap probabilities by both maximum parsimony and neighbor-joining methods. By contrast, mitochondrial protein genes coding for cytochrome b and cytochrome oxidase subunit I did not reconstruct the established phylogeny or did so only weakly, although a variety of fractions of the protein gene sequences were subjected to tree-building. This discouraging phylogenetic performance of mitochondrial protein genes, especially with respect to branches originating over 300 Myr ago, was not simply due to high randomness in the data. It may have been due to the relative susceptibility of the protein genes to natural selection as compared with the stem parts of mitochondrial tRNA genes. On the basis of these results, it is proposed that mitochondrial tRNA genes may be useful in resolving deep branches in animal phylogenies with divergences that occurred some hundreds of Myr ago. For this purpose, we designed a set of primers with which mtDNA fragments encompassing clustered tRNA genes were successfully amplified from various vertebrates by the polymerase chain reaction.

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Complete mitochondrial genome of the Korean ikonnikov's bat Myotis ikonnikovi (Chiroptera: Vespertilionidae).

Kwang Yoon, Jae Cho, Yung Park (2015)

We have characterized the complete mitochondrial genome of a Korean ikonnikov's bat species, Myotis ikonnikovi. Total length of the mitogenome is 16,585 bp with a total base composition of 34.0% A, 30.4% T, 22.8% C and 12.8% G. The mitogenome consists of 13 protein-coding genes, 2 rRNA (12S and 16S RNA) genes, 22 tRNA genes and 1 control region. The mitogenome of M. ikonnikovi exhibited similar features compared with the previously determined mitogenome of a Myotis species, M. formosus. The results will contribute to our understanding of the mechanisms underlying mitogenome evolution in the genus Myotis bats.

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Journal

Journal ID (nlm-ta): Mitochondrial DNA B Resour

Journal ID (iso-abbrev): Mitochondrial DNA B Resour

Title: Mitochondrial DNA. Part B, Resources

Publisher: Taylor & Francis

ISSN (Electronic): 2380-2359

Publication date (Electronic, pub): 11 May 2018

Publication date (Electronic, collection): 2018

Volume: 3

Issue: 2

Pages: 570-571

Affiliations

[a ]Department of Life Science, Dongguk University , Gyeongju, South Korea;

[b ]National Institute of Biological Resources , Inheon, South Korea;

[c ]Nakdonggang National Institute of Biological Resources , Sangju-si, South Korea

Author notes

CONTACT Jeong-Nam Yu susia000@ 123456nnibr.re.kr Nakdonggang National Institute of Biological Resources , Sangju-si37242, South Korea

Article

Publisher ID: 1467740

DOI: 10.1080/23802359.2018.1467740

PMC ID: 7800152

SO-VID: 31d8562b-cd1a-43f2-a6ce-b8d4cb414b7f

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This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License ( http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

The complete mitochondrial genome of long-tailed whiskered bat, Myotis frater (Myotis, Vespertilionidae)

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Genome Integrity

Most cited references 4

tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.

Sequence evolution of mitochondrial tRNA genes and deep-branch animal phylogenetics.

Complete mitochondrial genome of the Korean ikonnikov's bat Myotis ikonnikovi (Chiroptera: Vespertilionidae).

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