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      Influence of Furosemide on Rubidium-86 Uptake and Alpha-Adrenergic Responsiveness of Arterial Smooth Muscle

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          Abstract

          Furosemide-induced inhibition of <sup>86</sup>Rb uptake was measured in rat and rabbit aorta and compared with its ability to inhibit contractions induced by α-adrenergic agonists. In both rat and rabbit tissues, furosemide defined a portion of <sup>86</sup>Rb uptake (IC<sub>50</sub> = 2.5 µ M) which was distinct from the ouabain-sensitivite fraction. Furosemide-sensitive <sup>86</sup>Rb uptake was [Cl<sup>–</sup>]<sub>ext</sub> dependent and required Na<sup>+</sup> and K<sup>+</sup> for optimal activity, suggesting that it reflected a Na<sup>+</sup>-K<sup>+</sup> cotransport process. Furosemide-sensitive <sup>86</sup>Rb uptake was found to be greater in HEPES buffer than in bicarbonate buffer. Phenylephrine-induced contractions of rat and rabbit aorta were inhibited by furosemide; however, rat responses were far more sensitive. Agonist-induced uptake of <sup>45</sup>Ca was reduced by furosemide in rat aorta, but not in rabbit aorta. Agonist-induced <sup>45</sup>Ca efflux stimulation was reduced in both species. These findings indicate the presence in arteries of a furosemide-sensitive, Cl<sup>–</sup> dependent Na<sup>+</sup>-K<sup>+</sup> cotransport process. Along with other monovalent transport processes, it may modulate Ca<sup>2+</sup> availability and thereby influence arterial contractility.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1987
          1987
          23 September 2008
          : 24
          : 6
          : 321-333
          Affiliations
          Section of Pharmacology, College of Pharmacy and Allied Health Professions, Northeastern University, Boston, Mass., USA
          Article
          158709 Blood Vessels 1987;24:321–333
          10.1159/000158709
          321237f2-b80a-443b-96d9-6d6b99a157b8
          © 1987 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 05 June 1986
          : 26 November 1986
          Page count
          Pages: 13
          Categories
          Research Paper

          General medicine,Neurology,Cardiovascular Medicine,Internal medicine,Nephrology
          Vascular smooth muscle,Potassium flux,Sodium-potassium cotransport,Ouabain insensitivity,Rabbit aorta,Furosemide

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