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      15 years of GDR: New data and functionality in the Genome Database for Rosaceae

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          Abstract

          The Genome Database for Rosaceae (GDR, https://www.rosaceae.org) is an integrated web-based community database resource providing access to publicly available genomics, genetics and breeding data and data-mining tools to facilitate basic, translational and applied research in Rosaceae. The volume of data in GDR has increased greatly over the last 5 years. The GDR now houses multiple versions of whole genome assembly and annotation data from 14 species, made available by recent advances in sequencing technology. Annotated and searchable reference transcriptomes, RefTrans, combining peer-reviewed published RNA-Seq as well as EST datasets, are newly available for major crop species. Significantly more quantitative trait loci, genetic maps and markers are available in MapViewer, a new visualization tool that better integrates with other pages in GDR. Pathways can be accessed through the new GDR Cyc Pathways databases, and synteny among the newest genome assemblies from eight species can be viewed through the new synteny browser, SynView. Collated single-nucleotide polymorphism diversity data and phenotypic data from publicly available breeding datasets are integrated with other relevant data. Also, the new Breeding Information Management System allows breeders to upload, manage and analyze their private breeding data within the secure GDR server with an option to release data publicly.

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          Most cited references35

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          Gene Ontology: tool for the unification of biology

          Genomic sequencing has made it clear that a large fraction of the genes specifying the core biological functions are shared by all eukaryotes. Knowledge of the biological role of such shared proteins in one organism can often be transferred to other organisms. The goal of the Gene Ontology Consortium is to produce a dynamic, controlled vocabulary that can be applied to all eukaryotes even as knowledge of gene and protein roles in cells is accumulating and changing. To this end, three independent ontologies accessible on the World-Wide Web (http://www.geneontology.org) are being constructed: biological process, molecular function and cellular component.
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            Consed: a graphical tool for sequence finishing.

            Sequencing of large clones or small genomes is generally done by the shotgun approach (Anderson et al. 1982). This has two phases: (1) a shotgun phase in which a number of reads are generated from random subclones and assembled into contigs, followed by (2) a directed, or finishing phase in which the assembly is inspected for correctness and for various kinds of data anomalies (such as contaminant reads, unremoved vector sequence, and chimeric or deleted reads), additional data are collected to close gaps and resolve low quality regions, and editing is performed to correct assembly or base-calling errors. Finishing is currently a bottleneck in large-scale sequencing efforts, and throughput gains will depend both on reducing the need for human intervention and making it as efficient as possible. We have developed a finishing tool, consed, which attempts to implement these principles. A distinguishing feature relative to other programs is the use of error probabilities from our programs phred and phrap as an objective criterion to guide the entire finishing process. More information is available at http:// www.genome.washington.edu/consed/consed. html.
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              Is Open Access

              JBrowse: a dynamic web platform for genome visualization and analysis

              Background JBrowse is a fast and full-featured genome browser built with JavaScript and HTML5. It is easily embedded into websites or apps but can also be served as a standalone web page. Results Overall improvements to speed and scalability are accompanied by specific enhancements that support complex interactive queries on large track sets. Analysis functions can readily be added using the plugin framework; most visual aspects of tracks can also be customized, along with clicks, mouseovers, menus, and popup boxes. JBrowse can also be used to browse local annotation files offline and to generate high-resolution figures for publication. Conclusions JBrowse is a mature web application suitable for genome visualization and analysis.
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                Author and article information

                Journal
                Nucleic Acids Res
                Nucleic Acids Res
                nar
                Nucleic Acids Research
                Oxford University Press
                0305-1048
                1362-4962
                08 January 2019
                24 October 2018
                24 October 2018
                : 47
                : Database issue , Database issue
                : D1137-D1145
                Affiliations
                [1 ]Department of Horticulture, Washington State University, Pullman, WA 99164-6414, USA
                [2 ]Department of Plant and Environmental Sciences, Clemson University, Clemson, SC 29634-0310, USA
                [3 ]Department of Agronomy and Plant Genetics, University of Minnesota, St Paul, MN 55108, USA
                [4 ]Department of Horticulture, Washington State University Tree Fruit Research and Extension Center, Wenatchee, WA 98801, USA
                [5 ]Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
                [6 ]Department of Horticulture, Washington State University, Northwestern Washington Research and Extension Center, Mount Vernon, WA 98273, USA
                [7 ]Cedar Lake Research Group, LLC, Portland, OR 97293, USA
                [8 ]Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT 06269, USA
                [9 ]Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, USA
                [10 ]Forest Health Research and Extension Center, University of Kentucky, Lexington, KY 40546-0091, USA
                Author notes
                To whom correspondence should be addressed. Tel: +1 509 335 2774; Fax: +1 509 335 8690; Email: dorrie@ 123456wsu.edu
                Author information
                http://orcid.org/0000-0003-4391-5262
                http://orcid.org/0000-0003-2299-570X
                http://orcid.org/0000-0003-2971-9353
                Article
                gky1000
                10.1093/nar/gky1000
                6324069
                30357347
                3225847e-0c38-4405-9f65-568d8f519cf3
                Published by Oxford University Press on behalf of Nucleic Acids Research 2018.

                This work is written by (a) US Government employee(s) and is in the public domain in the US.

                History
                : 09 October 2018
                : 01 September 2018
                Page count
                Pages: 9
                Funding
                Funded by: National Institute of Food and Agriculture 10.13039/100005825
                Award ID: 2014-51181-2237
                Award ID: 2014-51181-22378
                Categories
                Database Issue

                Genetics
                Genetics

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