Sirt1 regulates apoptosis and extracellular matrix degradation in resveratrol‑treated osteoarthritis chondrocytes via the Wnt/β‑catenin signaling pathways
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Abstract
<p class="first" id="d3204568e131">Osteoarthritis (OA) has become a major public health
problem with the increased aging
population. Previous studies have demonstrated that resveratrol (RES) was able to
increase the level of sirtuin 1 (Sirt1) in OA chondrocytes. However, further investigations
are required to elucidate the precise molecular mechanism of RES and the potential
link between Sirt1 and RES. Therefore, the present study used 30 clinical OA chondrocyte
to examine chondrocyte viability, apoptosis rate and the mRNA and protein expression
levels of Sirt1 and relevant genes implicated in apoptosis, extracellular matrix (ECM)
degradation and Wnt/β-catenin signaling pathway. RES and nicotinamide were used as
the stimulus and inhibitor, respectively. The results demonstrated that the apoptotic
rate reduced as the cell population decreased from 13.83 to 6.55% in response to 10
µM RES. Expression levels of B-cell lymphoma 2 (Bcl-2) associated X protein (Bax),
procaspase-3 and −9, matrix metalloproteinase 1 (MMP1), MMP3, MMP13, Wnt3a, Wnt5a,
Wnt7a and β-catenin were significantly inhibited (P<0.01), whereas the level of
Bcl-2
was significantly increased (P<0.01) in OA chondrocytes treated with 10 µM RES.
These
observations suggested that Sirt1 may regulate apoptosis and ECM degradation in RES-treated
osteoarthritis chondrocytes via the Wnt/β-catenin signaling pathway.
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