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      Climate influence on chemical composition and antioxidant activity of Justicia pectoralis Jacq. Translated title: Influencia del clima en la composición química y la actividad antioxidante de Justicia pectorales Jacq

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          Abstract

          In this paper, the influence of the altitude in the phenols and flavonoids contents and the antioxidant activity of the extracts of different polarities of Justicia pectoralis Jacq(Acanthaceae) was evaluated. There was found that the plants cultivated under the Ibagué-Tolima's climate and soil conditions, can be a source of antioxidant compounds, especially in water preparations. The chromatographic analysis revealed that J. pectoralis extracts have mainly flavonoids of the flavonone type, as apigenine. A greater (or The greatest) content of flavonoids was detected in the ethanolic extract of the plant samples grown at 1 265 m.a.s.l. (meters above sea level) (2 748,03 mg/L). The altitude level does not seem to have any influence on the functional properties, neither on the phytophenols content. The J. pectoralis Jacq can be considered one species with a high therapeutic potential and with good commercial opportunities.

          Translated abstract

          En este trabajo se evaluó la influencia de la altitud en el contenido de fenoles y flavonoides y la actividad antioxidante de los extractos de diferentes polaridades provenientes de Justicia pectorales (Acanthaceae). Se halló que las plantas cultivadas en las condiciones del suelo y el clima de la zona Ibagué-Tolima pueden constituirse en compuestos antioxidantes, especialmente en preparados acuosos. El análisis cromatográfico reveló que los extractos de J. pectorales poseen en lo fundamental flavonoides del tipo flavona como es la apigenina. Un mayor contenido (sino el mayor) de flavonoides se detectó en el extracto etanol obtenido de las muestras de la planta cultivadas a 1 265 metros por encima del nivel del mar (2 748,03 mg/L). La altitud no parece influir en las propiedades funcionales, ni en el contenido de fitofenoles del extracto. La J. pectorales puede considerarse una especie de gran potencial terapéutico y buenas posibilidades de comercialización.

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          Most cited references31

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          Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents

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            Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea.

            An antioxidant fraction of Chinese green tea (green tea antioxidant; GTA), containing several catechins, has been previously shown to inhibit 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced tumor promotion in mouse skin. In the present study, GTA was shown to have antioxidative activity toward hydrogen peroxide (H2O2) and the superoxide radical (O2-). GTA also prevented oxygen radical and H2O2-induced cytotoxicity and inhibition of intercellular communication in cultured B6C3F1 mouse hepatocytes and human keratinocytes (NHEK cells). GTA (0.05-50 micrograms/ml) prevented the killing of hepatocytes (measured by lactate dehydrogenase release) by paraquat (1-10 mM) and glucose oxidase (0.8-40 micrograms/ml) in a concentration-dependent fashion. GTA (50 micrograms/ml) also prevented the inhibition of hepatocyte intercellular communication by paraquat (5 mM), glucose oxidase (0.8 micrograms/ml), and phenobarbital (500 micrograms/ml). In addition, GTA (50 micrograms/ml) prevented the inhibition of intercellular communication in human keratinocytes by TPA (100 ng/ml). Cytotoxicity and inhibition of intercellular communication, two possible mechanisms by which tumor promoters may produce their promoting effects were therefore prevented by GTA. The inhibition of these two effects of pro-oxidant compounds may suggest a mechanism by which GTA inhibits tumor promotion in vivo.
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              Reactive oxygen species, antioxidants, and the mammalian thioredoxin system.

              Reactive oxygen species (ROS) are known mediators of intracellular signaling cascades. Excessive production of ROS may, however, lead to oxidative stress, loss of cell function, and ultimately apoptosis or necrosis. A balance between oxidant and antioxidant intracellular systems is hence vital for cell function, regulation, and adaptation to diverse growth conditions. Thioredoxin reductase (TrxR) in conjunction with thioredoxin (Trx) is a ubiquitous oxidoreductase system with antioxidant and redox regulatory roles. In mammals, extracellular forms of Trx also have cytokine-like effects. Mammalian TrxR has a highly reactive active site selenocysteine residue resulting in a profound reductive capacity, reducing several substrates in addition to Trx. Due to the reactivity of TrxR, the enzyme is inhibited by many clinically used electrophilic compounds including nitrosoureas, aurothioglucose, platinum compounds, and retinoic acid derivatives. The properties of TrxR in combination with the functions of Trx position this system at the core of cellular thiol redox control and antioxidant defense. In this review, we focus on the reactions of the Trx system with ROS molecules and different cellular antioxidant enzymes. We summarize the TrxR-catalyzed regeneration of several antioxidant compounds, including ascorbic acid (vitamin C), selenium-containing substances, lipoic acid, and ubiquinone (Q10). We also discuss the general cellular effects of TrxR inhibition. Dinitrohalobenzenes constitute a unique class of immunostimulatory TrxR inhibitors and we consider the immunomodulatory effects of dinitrohalobenzene compounds in view of their reactions with the Trx system.
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                Author and article information

                Journal
                far
                Revista Cubana de Farmacia
                Rev Cubana Farm
                Editorial Ciencias Médicas (Ciudad de la Habana, , Cuba )
                0034-7515
                1561-2988
                March 2011
                : 45
                : 1
                : 88-100
                Affiliations
                [01] Ibagué-Tolima orgnameDepartament of Chemistry -University of Tolima Colombia jmendez@ 123456ut.edu.co
                [02] Pereira-Risaralda orgnameSchool of Technological Chemistry-University Thecnological of Pereira Colombia
                Article
                S0034-75152011000100010 S0034-7515(11)04500110
                3261755f-835b-4ab2-a0eb-3362f1538caa

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 05 October 2010
                : 13 November 2010
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 28, Pages: 13
                Product

                SciELO Cuba

                Self URI: Full text available only in PDF format (EN)
                Categories
                PRODUCTOS NATURALES

                Antioxidant activity,flavonoides,contenido de fenoles,Justicia pectorales (Acanthaceae),Justicia pectoralis (Acanthaceae),phenolic content,Flavonoids,actividad antioxidante

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