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      Use of biotechnologies for the conservation of plant biodiversity

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      Journal: In Vitro Cellular & Developmental Biology - Plant
      Publisher: Springer Nature

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          Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification.

          A Sakai, I Oiyama, Koichi Kobayashi (1990)
          The nucellar cells of navel orange(Citrus sinensis Osb. var. brasiliensis Tanaka) were successfully cryopreserved by vitrification. In this method, cells were sufficiently dehydrated with highly concentrated cryoprotective solution(PVS2) prior to direct plunge in liquid nitrogen. The PVS2 contains(w/v) 30% glycerol, 15% ethylene glycol and 15% DMSO in Murashige-Tucker medium(MT) containing 0.15 M sucrose. Cells were treated with 60% PVS2 at 25°C for 5 min and then chilled PVS2 at 0°C for 3 min. The cell suspension of about 0.1 ml was loaded in a 0.5 ml transparent plastic straw and directly plunged in liquid nitrogen for 30 min. After rapid warming, the cell suspension was expelled in 2 ml of MT medium containing 1.2 M sucrose. The average rate of survival was about 80%. The vitrified cells regenerated plantlets. This method is very simple and the time required for cryopreservation is only about 10 min.
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            Vitrification as an approach to cryopreservation

            G.M. Fahy, D.R. MacFarlane, C.A. Angell … (1984)
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              Plant cryopreservation: Progress and prospects

              Florent Engelmann (2004)
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                Author and article information

                Journal
                Title: In Vitro Cellular & Developmental Biology - Plant
                Abbreviated Title: In Vitro Cell.Dev.Biol.-Plant
                Publisher: Springer Nature
                ISSN (Print): 1054-5476
                ISSN (Electronic): 1475-2689
                Publication date Created: February 2011
                Publication date (Print): November 2010
                Volume: 47
                Issue: 1
                Pages: 5-16
                Article
                DOI: 10.1007/s11627-010-9327-2
                SO-VID: 3294234b-4531-4005-a63b-f80d28c6ec33
                Copyright © © 2011
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