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      Integration of a 3D-printed read-out platform with a quantum dot-based immunoassay for detection of the avian influenza A (H7N9) virus.

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          Abstract

          Outbreaks and potential epidemics of the highly pathogenic avian influenza virus pose serious threats to human health and the global economy. As such, its timely and accurate detection is critically important. In the present study, positive hybridoma cells (6B3) were obtained, which were used to secrete high-titer avian influenza virus (AIV) H7N9 monoclonal antibodies (H7N9 mAb). Based on these mAbs, quantum dot-based lateral flow immunochromatographic strips (QD-LFICS) were developed for AIV H7N9 detection. Under optimized conditions, results from a commercial fluorescent strip reader indicated that the limit of detection of QD-LFICS was 0.0268 HAU. To achieve rapid on-site testing, a mini 3D-printed read-out platform was fabricated to allow observation of QD-LFICS by the naked eye. More importantly, QD-LFICS were found to be practical and specific for the detection of actual samples compared with a real-time polymerase chain reaction.

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          Author and article information

          Journal
          Analyst
          The Analyst
          Royal Society of Chemistry (RSC)
          1364-5528
          0003-2654
          Apr 08 2019
          : 144
          : 8
          Affiliations
          [1 ] Department of Bioengineering, Guangdong Province Engineering Research Center for antibody drug and immunoassay, Jinan University, Guangzhou 510632, PR China. tyjaq7926@163.com.
          Article
          10.1039/c8an02336k
          30830133
          32a540b7-b7d7-4c3a-a0c8-6919bbe9bf42
          History

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