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      Two-color immunofluorescence using a fluorescence-activated cell sorter.

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          Abstract

          A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.

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          Author and article information

          Journal
          J. Histochem. Cytochem.
          The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
          SAGE Publications
          0022-1554
          0022-1554
          Jul 1977
          : 25
          : 7
          Article
          10.1177/25.7.330738
          330738
          32a7b993-8289-470e-b473-ba744b644909
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