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      Buffer combinations for mammalian cell culture.

      Science (New York, N.Y.)

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          Abstract

          The growth and metabolism of cultured mammalian cells are markedly affected by the pH variation in ordinary hicarbonate-buffered media (pH 8.0 to 6.9). Those pH swings can be reduced and the pH of the culture can be stabilized as desired in the range pH 6.4 to 8.3 by appropriate combinations of two or three organic buffers, each at 10 to 15 millimolar, in conjunction with phosphate and bicarbonate. The initial alkalinization in sparse cultures is then minimized, and the metabolic acidificatiotn in 24 hours is usually less than 0.4 pH unit except in heavy cultutres.

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          Author and article information

          Journal
          Science
          Science (New York, N.Y.)
          0036-8075
          0036-8075
          Oct 29 1971
          : 174
          : 4008
          Article
          10.1126/science.174.4008.500
          5110427
          32bef117-c4f6-47eb-9480-4aecd3a80aee
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