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Radiolabeled annexin V imaging: diagnosis of allograft rejection in an experimental rodent model of liver transplantation.

Radiology

Acute Disease, Animals, Annexin A5, diagnostic use, Graft Rejection, immunology, radionuclide imaging, Imino Acids, Liver, Liver Transplantation, Male, Organotechnetium Compounds, Radiopharmaceuticals, Rats, Rats, Inbred Lew, Rats, Inbred Strains, Sensitivity and Specificity, Technetium Tc 99m Aggregated Albumin

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      Abstract

      To assess the value of imaging rejection-induced apoptosis with technetium 99m and annexin V, a human protein-based radiopharmaceutical used in the diagnosis of acute rejection of a liver transplant, in a well-characterized rodent model of orthotopic liver transplantation. 99mTc-radiolabeled annexin V was intravenously administered to six allografted (immunologically mismatched) and five isografted (immunologically matched) recipient rats on days 2, 4, and 7 after orthotopic liver transplantation. Animals were imaged 1 hour after injection of 0.2-2.0 mCi (8.0-74.0 MBq) of radiolabeled annexin V by use of clinical nuclear scintigraphic equipment. All animals in the allografted group demonstrated marked increases of 55% and 97% above the activity in the isografted group in hepatic uptake of annexin V on days 4 and 7, respectively. Severe acute rejection was histologically detected in all allografted livers on day 7. There was no histologic evidence of acute rejection in isografted animals. Dynamic hepatobiliary imaging with 99mTc and mebrofenin, an iminodiacetic acid derivative, demonstrated no correlation with the presence or absence of acute rejection or with annexin V uptake. Noninvasive imaging with radiolabeled annexin V is more sensitive and specific than imaging with 99mTc-mebrofenin in the diagnosis of acute rejection of a liver transplant.

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      10715048
      10.1148/radiology.214.3.r00mr34795

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