We here treat four unreported or poorly known mayfly species from Jordan: Cheleocloeon soldani Gattolliat & Sartori, 2008, Cloeon vanharteni Gattolliat & Sartori, 2008, Baetis pacis Yanai & Gattolliat, 2018, and Caenis macrura Stephens, 1835. They are reported and morphologically distinguished from other local relatives. Caenis macrura is widely distributed in the Palaearctic, Ch. soldani and C. vanharteni originated from the Arabian Peninsula, and B. pacis is restricted to a small area in southern Levant. We provide a complete checklist of the Jordanian mayfly fauna and deliver an identification key to the nymphal stages.