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      Evaluación de diecinueve accesiones de Leucaena leucocephala basada en la calidad nutritiva del forraje Translated title: Evaluation of nineteen accessions of Leucaena leucocephala based in the nutritive quality of forage


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          Se llevó a cabo un experimento para evaluar, mediante el análisis de componentes principales (ACP) y de clasificación automática (ACA), las variaciones de la composición química e indicadores de valor nutritivo en el follaje de diecinueve accesiones de Leucaena leucocephala Lam. de Wit establecidas en Matanzas, Cuba. Se determinó la composición bromatológica, los niveles de metabolitos secundarios y la degradabilidad ruminal in situ de la MS, PC y FDN. Mediante el ACP se detectó una variabilidad discreta para los primeros tres componentes(48,87%). Los indicadores que explicaron mejor las variaciones entre las accesiones fueron los niveles de MS, PC, PV, FDN, FDA, FC, LDA, celulosa, Ca, cenizas, taninos condensados (TC) y degradabilidad de la MS. Sin embargo, la concentración de P, K, polifenoles totales, taninos precipitantes, fitatos, mimosina y la degradabilidad ruminal de la PC y FDN no constituyeron variables fidedignas para comparar las accesiones evaluadas en las condiciones experimentales. Mediante el ACA se identificaron cinco grupos con características distintivas entre sí. Aunque todas las accesiones presentaron buena composición química y valor nutritivo las accesiones de mejores resultados fueron CIAT-9438, CIAT-8069, CIAT-7985, CIAT-9119, CIAT-9993, CIAT-7929 y CIAT-937. Solamente los TC constituyen metabolitos secundarios con importancia comparativa.

          Translated abstract

          An experiment was carried out in order to evaluate, using the main components (ACP) and automatic classification analysis (ACA), the chemical composition and nutritional value variations of nineteen accessions of Leucaena leucocephala Lam. de Wit foliage in Matanzas, Cuba. Bromatological composition, secondary metabolites level and in situ ruminal degradabilidad (DM, CP and NDF) were determined. A discreet variability for the first three components (48,87 %) were detected. The DM, CP, TP, NDF, ADF, CF, ADL, cellulose, Ca, ashes and condensed tannins (CT) levels and DM ruminal degradability explained the variability among the evaluate accessions. However, under experimental conditions the P, K, total polyphenols, precipitant tannins, phytates and mimosine concentration, and CP and NDF ruminal degradability constitute marginal variables to compare the accessions. Using the ACA in dependence of the nutritional profile five groups with distinctive characteristic to each other were identified. The studied accessions differ fundamentally for their proximal composition and the CIAT-9438, CIAT-8069, CIAT-7985, CIAT-9119, CIAT-9993, CIAT-7929 y CIAT-937 accessions showed the best results. Only the CT constitutes the group of secondary metabolites with comparative importance.

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          Most cited references43

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          A critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain

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            Enhancing the thermal tolerance and gastric performance of a microbial phytase for use as a phosphate-mobilizing monogastric-feed supplement.

            The inclusion of phytase in monogastric animal feed has the benefit of hydrolyzing indigestible plant phytate (myo-inositol 1,2,3,4,5,6-hexakis dihydrogen phosphate) to provide poultry and swine with dietary phosphorus. An ideal phytase supplement should have a high temperature tolerance, allowing it to survive the feed pelleting process, a high specific activity at low pHs, and adequate gastric performance. For this study, the performance of a bacterial phytase was optimized by the use of gene site saturation mutagenesis technology. Beginning with the appA gene from Escherichia coli, a library of clones incorporating all 19 possible amino acid changes and 32 possible codon variations in 431 residues of the sequence was generated and screened for mutants exhibiting improved thermal tolerance. Fourteen single site variants were discovered that retained as much as 10 times the residual activity of the wild-type enzyme after a heated incubation regimen. The addition of eight individual mutations into a single construct (Phy9X) resulted in a protein of maximal fitness, i.e., a highly active phytase with no loss of activity after heating at 62 degrees C for 1 h and 27% of its initial activity after 10 min at 85 degrees C, which was a significant improvement over the appA parental phytase. Phy9X also showed a 3.5-fold enhancement in gastric stability.
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              Determination of Both Tannin and Protein in a Tannin-Protein Complex


                Author and article information

                Zootecnia Tropical
                Zootecnia Trop.
                Instituto Nacional de Investigaciones Agricolas INIA, Maracay, Venezuela. (Maracay, Aragua, Venezuela )
                March 2008
                : 26
                : 1
                : 9-18
                [01] Trujillo Trujillo orgnameFundación para el Desarrollo de la Ciencia y la Tecnología del estado Trujillo Venezuela
                [03] Trujillo Trujillo orgnameUniversidad de los Andes orgdiv1Núcleo Universitario Rafael Rangel Venezuela
                [04] Pampanito Trujillo orgnameInstituto Nacional de Investigaciones Agrícolas orgdiv1Estación Experimental de Pampanito Venezuela
                [05] La Habana orgnameUniversidad de la Habana orgdiv1Facultad de Química Cuba
                [02] Matanzas orgnameEstación Experimental de Pastos y Forrajes Indio Hatuey Cuba
                S0798-72692008000100002 S0798-7269(08)02600102


                : 07 May 2007
                : 29 January 2008
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 35, Pages: 10

                SciELO Venezuela

                Self URI: Texto completo solamente en formato PDF (ES)
                Artículos Científicos

                Leucaena leucocephala,forraje,composición química,valor nutritivo,metabolitos secundarios,taninos,forage,chemical composition,nutritive value,secondary metabolites tannins


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