0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      PGE 2 Synthesis by Corneal Endothelial Cells: Effect of Glucocorticoids and NSAIDs

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The aim of this work was to study the effect of various anti-inflammatory drugs on PGE<sub>2</sub> synthesis in cultured bovine corneal endothelial cells (BCECs) stimulated with calcium ionophore A<sub>23187</sub> or lipopolysaccharide (LPS) of Salmonella typhimurium. NSAIDs were more potent in inhibiting LPS-stimulated PGE<sub>2</sub> synthesis. Diclofenac was more potent than indomethacin, although both drugs showed a 98% maximal inhibitory effect. Dexamethasone inhibited 80% of the A<sub>23187</sub>-stimulated PGE<sub>2</sub> synthesis and only 53% of the LPS-stimulated PGE<sub>2</sub> synthesis. Prednisolone did not show an inhibitory effect. The results demonstrate the inhibitory effect of NSAIDs and show differences between the activity of glucocorticoids on PGE<sub>2</sub> synthesis in BCECs. Prednisolone could not inhibit PGE<sub>2</sub> synthesis in these cells in our experimental conditions.

          Related collections

          Most cited references 4

          • Record: found
          • Abstract: not found
          • Article: not found

          Cyclooxygenase-2: regulation and relevance in inflammation.

            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Anti-inflammatory actions of steroids: molecular mechanisms

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Proinflammatory cytokines regulate cyclooxygenase-2 mRNA expression in human macrophages.

              Prostaglandins play a major role in the inflammatory and immune response. Macrophages constitutively produce prostaglandins by the enzyme cyclooxygenase-1 (cox) whereas inflammatory mediators and cytokines stimulate the inducible enzyme cox-2 for high output prostaglandin production. In this study, we investigated the effect of LPS, IFN-gamma and TNF-alpha in the regulation of cox-1 and cox-2 mRNA expression in PMA-differentiated U937 human macrophages. LPS, but not IFN-gamma or TNF-alpha, caused the induction of cox-2 mRNA in a dose- and time-dependent fashion. In IFN-gamma-primed macrophages, LPS stimulated a marked increase in the accumulation of cox-2 mRNA, whereas TNF-alpha and IFN-gamma induced a moderate level. However, IFN-gamma in combination with either LPS or TNF-alpha induced a synergistic increase in the accumulation of cox-2 mRNA after 24 hours. Regardless of the conditions, cox-1 mRNA remained unchanged. These results indicate that IFN-gamma priming is required for full expression of cox-2 mRNA in response to LPS or TNF-alpha stimulation and suggest that cox-2 mRNA is highly regulated by cytokines during macrophage activation.
                Bookmark

                Author and article information

                Journal
                ORE
                Ophthalmic Res
                10.1159/issn.0030-3747
                Ophthalmic Research
                S. Karger AG
                0030-3747
                1423-0259
                1999
                February 1999
                02 December 1998
                : 31
                : 1
                : 42-46
                Affiliations
                Departamento I de Optica, Universidad de Alicante, España
                Article
                55511 Ophthalmic Res 1999;31:42–46
                10.1159/000055511
                9831821
                © 1998 S. Karger AG, Basel

                Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

                Page count
                Figures: 1, Tables: 2, References: 16, Pages: 5
                Categories
                Original Paper

                Comments

                Comment on this article