The number of young people aged between 13 to 29 infected with Human Immunodeficiency Virus (HIV) was rapidly increasing in Malaysia. The objective of this study is to determine the level of knowledge, attitude, and perception among students in the University. This study employed the cross-sectional design. The questionnaire was distributed to 372 respondents. Therefore, 52.4 percent of students had good knowledge. There was a weak positive correlation between KA (r =0.23) and KP (r=0.19) with significance (p<0.05). Conclusion, an increase in the level of knowledge contributed to the positive attitude and perception also reduce the stigma and discrimination toward people living with HIV/AIDS.